Serotonin Receptor 3A antibody (AA 21-36)

Catalog No. ABIN297638

The Rabbit Polyclonal anti-Serotonin Receptor 3A antibody has been validated for WB, IHC and IHC (p). It is suitable to detect Serotonin Receptor 3A in samples from Human and Rat.

Quick Overview for Serotonin Receptor 3A antibody (AA 21-36) (ABIN297638)

Target: Serotonin Receptor 3A (HTR3A)

Reactivity: Human, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Serotonin Receptor 3A antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-Serotonin Receptor 3A Antibody

Binding Specificity: AA 21-36

Brand: IHC-plus™

Specificity: In human brain, a 47-50 kD band is observed.

Predicted Reactivity: Percent identity by BLAST analysis: Rat (100%) Mouse (88%) Hamster (81%).

Purification: Protein G purified

Immunogen: This antibody was developed by immunizing rabbits with a mixture of synthetic peptides corresponding to amino acids 21-36 (RATQAHSTTQPALLRL) and 421-436 (LSSIRHSLEKRDEMRE) of rat 5-HT3A receptor, GenBank no AAB02949. Percent identity by BLAST analysis: Rat (100%), Mouse (88%), Hamster (81%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: IHC, IHC-P (5 μg/mL), WB (1 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL.

Comment:

Target Species of Antibody: Rat

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS, 0.05 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for Serotonin Receptor 3A

Target: Serotonin Receptor 3A (HTR3A)

Alternative Name: HTR3A / 5-HT3A Receptor

Background: Name/Gene ID: HTR3A
Subfamily: Serotonin 5-HT3 receptor
Family: Ion channel

Synonyms: HTR3A, 5-HT3-A, 5-HT3R-A, 5-HT3 receptor, 5HT3 serotonin receptor, 5-HT3A, 5-HT3a receptor, 5-hydroxytryptamine receptor 3, 5-HT-3, 5-HT3, 5-HT3R, 5HT3R, HTR3, Serotonin receptor 3A

Gene ID: 3359

UniProt: P46098

Pathways: Synaptic Membrane