Mouse anti-Human IgG (Fc Region) Antibody (HRP)

Details for Product No. ABIN302063, Supplier: Log in to see
Antigen
Epitope
Fc Region
5120
1884
1842
1055
1039
866
330
304
35
34
31
12
8
7
3
3
2
1
1
1
1
1
1
1
Reactivity
Human
2913
2617
2172
1556
1393
751
601
456
426
389
365
357
295
280
265
145
132
113
41
30
29
26
16
11
10
8
7
6
5
5
4
4
4
3
3
3
3
3
3
2
2
2
1
1
1
1
1
Host
Mouse
6502
4930
1660
1008
521
357
93
67
54
19
8
5
5
5
3
1
1
Clonality (Clone)
Monoclonal ()
Conjugate
HRP
2221
1825
1740
1284
772
695
447
307
219
171
165
141
137
129
110
107
77
75
71
65
65
56
55
54
54
53
53
53
53
53
53
52
50
40
37
35
24
19
18
18
17
15
15
15
14
14
14
14
12
12
11
10
10
10
10
10
9
9
9
9
9
8
8
8
8
6
6
6
6
6
6
5
5
5
5
5
4
4
4
4
4
4
4
4
4
4
4
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
Application
ELISA, Western Blotting (WB)
Options
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Immunogen Fusion protein of human IgG Fc fragment.
Clone EM-07
Isotype IgG1
Specificity The antibody EM-07 reacts with Fc part of human IgG heavy chain and with isolated Fc fragments.
Characteristics The purified antibody is conjugated with Horseradish Peroxidase (HRP) of high specific activity and RZ=3.
Background Immunoglobulin G (IgG) is a 150 kDa soluble protein that serves as a major effector molecule of the humoral immune response in man. Its concentration in blood plasma of healthy individuals is approximately 10 g/l, which accounts for about 75 % of the total plasma immunoglobulins. IgG has the highest stability of blood immunoglobulins (T1/2 = 21 days) and is able of placental transfer. IgG is secreted by plasma cells at a comparably high rate as other immunoglobulins.
Molecular Weight 150 kDa
Application Notes The peroxidase conjugate is designed for ELISA and Western Blotting. Suggested working dilution for ELISA is 0.1 - 0.5 µg/mL. Indicated dilution is recommended starting point for use of this product. Working concentrations should be determined by the investigator.

Working concentrations should be determined by the investigator.
Restrictions For Research Use only
Format Liquid
Concentration 1 mg/mL
Buffer The reagent is provided in phosphate buffered saline (PBS) containing 0.01 % (w/v) thimerosal and 1 % (w/v) high-grade protease free Bovine Serum Albumin (BSA) as a stabilizing agent.
Preservative Thimerosal (Merthiolate)
Precaution of Use Biohazard Informations: This product contains thimerosal which is hazardous.
Handling Advice Do not freeze.
Avoid prolonged exposure to light.
Storage 4 °C
Storage Comment Store in the dark at 2-8 °C. Do not use after expiration date stamped on vial label. Short-term exposure to room temperature should not affect the quality of the reagent. However, if reagent is stored under any conditions other than those specified, the conditions must be verified by the user.
Supplier Images
Western Blotting (WB) image for Mouse anti-Human IgG (Fc Region) antibody (HRP) (ABIN302063) IgG κ light chain (1), IgG λ light chain (2) and IgG Fc fragment (3) purifi...
Background publications Brinkmann, Heusser: "T cell-dependent differentiation of human B cells into IgM, IgG, IgA, or IgE plasma cells: high rate of antibody production by IgE plasma cells, but limited clonal expansion of IgE precursors." in: Cellular immunology, Vol. 152, Issue 2, pp. 323-32, 1994 (PubMed).

Balogh, Merétey, Falus, Bozsóky: "Serological abnormalities in juvenile chronic arthritis: a review of 46 cases." in: Annals of the rheumatic diseases, Vol. 39, Issue 2, pp. 129-34, 1980 (PubMed).

Franklin: "Structure and function of immunoglobulins." in: Acta endocrinologica. Supplementum, Vol. 194, pp. 77-95, 1975 (PubMed).

Fuller, Keyser: "Serum immunoglobulins after surgical operation." in: Clinical chemistry, Vol. 21, Issue 6, pp. 667-71, 1975 (PubMed).