FEN1 antibody (AA 4-300)

Catalog No. ABIN7601619

This Mouse Monoclonal antibody specifically detects FEN1 in WB, IHC, IF, FACS and ICC. It exhibits reactivity toward Human and Mouse.

Quick Overview for FEN1 antibody (AA 4-300) (ABIN7601619)

Target: FEN1 (Flap Structure-Specific Endonuclease 1 (FEN1))

Reactivity: Human, Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This FEN1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS), Immunocytochemistry (ICC)

Clone: 7D11D7

Product Details anti-FEN1 Antibody

Binding Specificity: AA 4-300

Purpose: Anti-FEN1 Antibody (monoclonal, 7D11D7)

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-FEN1 Antibody.(monoclonal, 7D11D7)-4. Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human FEN1 recombinant protein (Position: Q4-E300).

Isotype: IgG2b

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human, Mouse Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/mL, Human Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for FEN1

Target: FEN1 (Flap Structure-Specific Endonuclease 1 (FEN1))

Alternative Name: FEN1

Background:

Background: Flap endonuclease 1 is an enzyme that in humans is encoded by the FEN1 gene. It is mapped to 11q12.2. The protein encoded by this gene removes 5' overhanging flaps in DNA repair and processes the 5' ends of Okazaki fragments in lagging strand DNA synthesis. physical interaction between this protein and AP endonuclease 1 during long-patch base excision repair provides coordinated loading of the proteins onto the substrate, thus passing the substrate from one enzyme to another. The protein is a member of the XPG/RAD2 endonuclease family and is one of ten proteins essential for cell-free DNA replication. DNA secondary structure can inhibit flap processing at certain trinucleotide repeats in a length-dependent manner by concealing the 5' end of the flap that is necessary for both binding and cleavage by the protein encoded by this gene. Therefore, secondary structure can deter the protective function of this protein, leading to site-specific trinucleotide expansions.

Gene Full Name: flap structure-specific endonuclease 1

Molecular Weight: 48 kDa

Gene ID: 2237

UniProt: P39748

Pathways: Telomere Maintenance, DNA Damage Repair, DNA Replication, Synthesis of DNA