p21 antibody (AA 43-142)

Catalog No. ABIN3022006

This anti-p21 antibody is a Rabbit Polyclonal antibody detecting p21 in WB and IF. Suitable for Human. This Primary Antibody has been cited in 3+ publications.

Quick Overview for p21 antibody (AA 43-142) (ABIN3022006)

Target: p21 (CDKN1A) (Cyclin-Dependent Kinase Inhibitor 1A (p21, Cip1) (CDKN1A))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This p21 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF)

Product Details anti-p21 Antibody

Binding Specificity: AA 43-142

Sequence: QEARERWNFD FVTETPLEGD FAWERVRGLG LPKLYLPTGP RRGRDELGGG RRPGTSPALL QGTAEEDHVD LSLSCTLVPR SGEQAEGSPG GPGDSQGRKR

Cross-Reactivity: Human, Mouse, Rat

Characteristics: Polyclonal Antibodies

Purification: Affinity purification

Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 43-142 of human CDKN1A/p21CIP1 (NP_000380.1).

Isotype: IgG

Application Details

Application Notes: WB,1:500 - 1:2000,IF,1:50 - 1:200

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: PBS with 0.02 % sodium azide,50 % glycerol, pH 7.3.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid freeze / thaw cycles

Storage: -20 °C

Storage Comment: Store at -20°C. Avoid freeze / thaw cycles.

References for anti-p21 antibody (ABIN3022006)

Guo, Zhao, Fei, Zhao, Zheng, Su, Wu, Li, Chang: "Dicer1 downregulation by multiple myeloma cells promotes the senescence and tumor-supporting capacity and decreases the differentiation potential of mesenchymal stem cells." in: Cell death & disease, Vol. 9, Issue 5, pp. 512, (2018) (PubMed).

Wu, Wang, Luo, Zhang, Wang, Zhu, Gao, Du, Yang: "Metabolic perturbation of epigenome by inhibiting S-adenosylhomocysteine hydrolase elicits senescence through DNA damage response in hepatoma cells." in: Tumour biology, Vol. 39, Issue 5, pp. 1010428317699117, (2017) (PubMed).

Li, Li, Li, Cui, Li, Guo, Han, Liu, Sun: "Involvement of Histone Lysine Methylation in p21 Gene Expression in Rat Kidney In Vivo and Rat Mesangial Cells In Vitro under Diabetic Conditions." in: Journal of diabetes research, Vol. 2016, pp. 3853242, (2017) (PubMed).

Target Details for p21

Target: p21 (CDKN1A) (Cyclin-Dependent Kinase Inhibitor 1A (p21, Cip1) (CDKN1A))

Alternative Name: CDKN1A

Background: This gene encodes a potent cyclin-dependent kinase inhibitor. The encoded protein binds to and inhibits the activity of cyclin-cyclin-dependent kinase2 or -cyclin-dependent kinase4 complexes, and thus functions as a regulator of cell cycle progression at G1. The expression of this gene is tightly controlled by the tumor suppressor protein p53, through which this protein mediates the p53-dependent cell cycle G1 phase arrest in response to a variety of stress stimuli. This protein can interact with proliferating cell nuclear antigen, a DNA polymerase accessory factor, and plays a regulatory role in S phase DNA replication and DNA damage repair. This protein was reported to be specifically cleaved by CASP3-like caspases, which thus leads to a dramatic activation of cyclin-dependent kinase2, and may be instrumental in the execution of apoptosis following caspase activation. Mice that lack this gene have the ability to regenerate damaged or missing tissue. Multiple alternatively spliced variants have been found for this gene.,CAP20,CDKN1,CIP1,MDA-6,P21,SDI1,WAF1,p21CIP1,CDKN1A,Epigenetics & Nuclear Signaling,DNA Damage & Repair,Cancer,Tumor suppressors,p53 pathway,Signal Transduction,Kinase,PI3K-Akt Signaling Pathway,Cell Biology & Developmental Biology,Apoptosis,Cell Cycle,Cell cycle inhibitors,G1/S Checkpoint,G2/M DNA Damage Checkpoint,Endocrine & Metabolism,AMPK Signaling Pathway,Immunology & Inflammation,Stem Cells,CDKN1A

Molecular Weight: 21 kDa

Gene ID: 1026

UniProt: P38936

Pathways: p53 Signaling, PI3K-Akt Signaling, Cell Division Cycle, AMPK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Mitotic G1-G1/S Phases, DNA Replication, Hepatitis C, Synthesis of DNA, Autophagy