KIT antibody (AA 100-300)

Catalog No. ABIN3023804

This Mouse Monoclonal antibody specifically detects KIT in WB, FACS, IF and IHC (fro). It exhibits reactivity toward Human.

Quick Overview for KIT antibody (AA 100-300) (ABIN3023804)

Target: KIT (Mast/stem Cell Growth Factor Receptor (KIT))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This KIT antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: KIT-982

Product Details anti-KIT Antibody

Binding Specificity: AA 100-300

Characteristics: This mAb recognizes a protein of 145 kDa, identified as CD117/p145kit/c-Kit. It is found on a wide variety of tumor cells including follicular and papillary carcinoma of thyroid, adenocarcinomas from endometrium, lung, ovary, pancreas, and breast as well as malignant melanoma, endodermal sinus tumor, and small cell carcinoma. However, anti-CD117/c-Kit has been particularly useful in differentiating gastrointestinal stromal tumors from Kaposi's sarcoma, tumors of smooth muscle origin, fibromatosis, and neural tumors of the GI tract. Anti-CD117/c-Kit is also useful in recognizing myeloblasts in bone marrow biopsy and clot section.

Purification: Protein G affinity chromatography

Immunogen: A recombinant human fragment around amino acids 100-300 was used as the immunogen for the c-Kit antibody.

Isotype: IgG1 kappa

Application Details

Application Notes: Optimal dilution of the c-Kit antibody should be determined by the researcher.\. Flow Cytometry: 0.5-1 μg/million cells in 0.1ml,Immunofluorescence: 0.5-1 μg/mL,Western blot (0.5-1 μg/mL for 2 hours at RT),Immunohistochemistry (Frozen only): 2-4 μg/mL for 30 min at RT

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: 1 mg/mL in 1X PBS, BSA free, sodium azide free

Preservative: Azide free

Storage: 4 °C,-20 °C

Storage Comment: Store the c-Kit antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).

Target Details for KIT

Target: KIT (Mast/stem Cell Growth Factor Receptor (KIT))

Alternative Name: C-Kit

Background: This mAb recognizes a protein of 145  kDa, identified as CD117/p145kit/c-Kit. It is found on a wide variety of tumor cells including follicular and papillary carcinoma of thyroid, adenocarcinomas from endometrium, lung, ovary, pancreas, and breast as well as malignant melanoma, endodermal sinus tumor, and small cell carcinoma. However, anti-CD117/c-Kit has been particularly useful in differentiating gastrointestinal stromal tumors from Kaposi's sarcoma, tumors of smooth muscle origin, fibromatosis, and neural tumors of the GI tract. Anti-CD117/c-Kit is also useful in recognizing myeloblasts in bone marrow biopsy and clot section.

Pathways: RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Sensory Perception of Sound, Stem Cell Maintenance, Production of Molecular Mediator of Immune Response, Regulation of long-term Neuronal Synaptic Plasticity