ZAP70 antibody (AA 1-254)

Catalog No. ABIN3024066

The Mouse Monoclonal anti-ZAP70 antibody is suitable to detect ZAP70 in samples from Human. It has been validated for WB, FACS, IF and IHC (p).

Quick Overview for ZAP70 antibody (AA 1-254) (ABIN3024066)

Target: ZAP70 (zeta-Chain (TCR) Associated Protein Kinase 70kDa (ZAP70))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This ZAP70 antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: 2F3-2

Product Details anti-ZAP70 Antibody

Binding Specificity: AA 1-254

Purpose: ZAP-70 Antibody

Purification: Protein G affinity chromatography

Immunogen: Recombinant human protein (including amino acids 1-254 and the SH2 domains) was used as the immunogen for the ZAP-70 antibody.

Isotype: IgG2a, kappa

Application Details

Application Notes: Optimal dilution of the ZAP-70 antibody should be determined by the researcher.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Tris with 1 mM EDTA, pH 9, for 10-20 min followed by cooling at RT for 20 min.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.2 mg/mL

Buffer: 0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store the ZAP-70 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Target Details for ZAP70

Target: ZAP70 (zeta-Chain (TCR) Associated Protein Kinase 70kDa (ZAP70))

Alternative Name: ZAP-70

Background: ZAP70 is a 70 kDa protein tyrosine kinase found in T-cells and natural killer cells. Control of this protein translation is via the IgVH gene. ZAP70 protein is expressed in leukemic cells of approximately 25 % of chronic lymphocytic leukemia (CLL) cases as well. Anti-ZAP70 expression is an excellent surrogate marker for the distinction between the Ig-mutated (anti-ZAP70 negative) and Ig-unmutated (anti-ZAP70 positive) CLL subtypes and can identify patient groups with divergent clinical courses. The anti-ZAP70 positive Ig-unmutated CLL cases have been shown to have a poorer prognosis.

UniProt: P43403

Pathways: TCR Signaling, Ubiquitin Proteasome Pathway