ZAP70 antibody (full length)

Catalog No. ABIN3024076

The Mouse Monoclonal anti-ZAP70 antibody (Clone ZAP70-528-2F3-2) (ABIN3024076) specifically detects ZAP70 in IHC (p). The antibody is reactive with Human samples.

Quick Overview for ZAP70 antibody (full length) (ABIN3024076)

Target: ZAP70 (zeta-Chain (TCR) Associated Protein Kinase 70kDa (ZAP70))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This ZAP70 antibody is un-conjugated

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Grade: Carrier-free

Clone: ZAP70-528-2F3-2

Product Details anti-ZAP70 Antibody

Binding Specificity: full length

Purpose: ZAP70 Antibody Cocktail (azide and preservative free)

Purification: Protein G affinity chromatography

Immunogen: Recombinant full-length human protein was used as the immunogen for the ZAP70 antibody cocktail.

Isotype: IgG2a, kappa

Application Details

Application Notes: Optimal dilution of the ZAP70 antibody cocktail should be determined by the researcher.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Tris with 1 mM EDTA, pH 9, for 10-20 min followed by cooling at RT for 20 min.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: 1 mg/mL in 1X PBS, BSA free, sodium azide free

Preservative: Azide free

Storage: 4 °C,-20 °C

Storage Comment: Store the ZAP70 antibody cocktail at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Target Details for ZAP70

Target: ZAP70 (zeta-Chain (TCR) Associated Protein Kinase 70kDa (ZAP70))

Alternative Name: ZAP70

Background: ZAP70 is a 70 kDa protein tyrosine kinase found in T-cells and natural killer cells. Control of this protein translation is via the IgVH gene. ZAP70 protein is expressed in leukemic cells of approximately 25 % of chronic lymphocytic leukemia (CLL) cases as well. Anti-ZAP70 expression is an excellent surrogate marker for the distinction between the Ig-mutated (anti-ZAP70 negative) and Ig-unmutated (anti-ZAP70 positive) CLL subtypes and can identify patient groups with divergent clinical courses. The anti-ZAP70 positive Ig-unmutated CLL cases have been shown to have a poorer prognosis.

UniProt: P43403

Pathways: TCR Signaling, Ubiquitin Proteasome Pathway