Melanoma gp100 antibody

Catalog No. ABIN3024237

The Mouse Monoclonal anti-Melanoma gp100 antibody is suitable to detect Melanoma gp100 in samples from Human. It has been validated for WB and IHC (p).

Quick Overview for Melanoma gp100 antibody (ABIN3024237)

Target: Melanoma gp100 (PMEL) (Premelanosome Protein (PMEL))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Melanoma gp100 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Grade: Carrier-free

Clone: HMB45-PMEL-783

Product Details anti-Melanoma gp100 Antibody

Purpose: gp100 Antibody Cocktail / PMEL17 (azide and preservative free)

Purification: Protein G affinity chromatography

Immunogen: Extract of pigmented melanoma metastases from lymph nodes (HMB45) and recombinant human protein (PMEL/783) were used as the immunogen for the gp100 antibody cocktail.

Isotype: IgG1, kappa

Application Details

Application Notes: Optimal dilution of the gp100 antibody cocktail should be determined by the researcher.
1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: 1 mg/mL in 1X PBS, BSA free, sodium azide free

Preservative: Azide free

Storage: 4 °C,-20 °C

Storage Comment: Store the gp100 antibody cocktail at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Target Details for Melanoma gp100

Target: Melanoma gp100 (PMEL) (Premelanosome Protein (PMEL))

Alternative Name: gp100

Background: By immunohistochemistry, it specifically recognizes a protein in melanocytes and melanomas. This mAb reacts with junctional and blue nevus cells and variably with fetal and neonatal melanocytes. Intradermal nevi, normal adult melanocytes, and non-melanocytic cells are negative. It does not stain tumor cells of epithelial, lymphoid, glial, or mesenchymal origin. Metastatic amelanotic melanoma can often be confused with a variety of poorly differentiated carcinomas, large cell lymphomas, and sarcomas using H & E stains alone. It is also difficult to differentiate melanoma from spindle cell carcinomas and various types of mesenchymal neoplasms. This mAb stains fetal and neonatal melanocytes, junctional and blue nevus cells, and malignant melanoma. This mAb also stains Angiomyolipoma (PEComa).

The gp100 Molecule is a 100 kDa glycosylated protein that is cleaved into a small (26 kDa) carboxy-terminal fragment and a larger amino- terminal section (60-64 kDa), which is subsequently cleaved to generate 26 kDa and 34-38 kDa.

UniProt: P40967