CD11b antibody

Catalog No. ABIN3024274

The Rat Monoclonal anti-CD11b antibody (Clone M1-70) (ABIN3024274) specifically detects CD11b in FACS, IF and Func. The antibody is reactive with Human and Mouse samples.

Quick Overview for CD11b antibody (ABIN3024274)

Target: CD11b (ITGAM) (Integrin alpha M (ITGAM))

Reactivity: Human, Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This CD11b antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunofluorescence (IF), Functional Studies (Func)

Grade: Carrier-free

Clone: M1-70

Product Details anti-CD11b Antibody

Purpose: CD11b Antibody / MAC-1 / ITGAM (azide and preservative free)

Purification: Protein G affinity chromatography

Immunogen: B10 mouse spleen cells enriched for T lymphocytes were used as the immunogen for this CD11b antibody.

Isotype: IgG2b, kappa

Application Details

Application Notes: The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the CD11b antibody to be titered up or down for optimal performance.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: 1 mg/mL in 1X PBS, BSA free, sodium azide free

Preservative: Azide free

Storage: 4 °C,-20 °C

Storage Comment: Store the CD11b antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Target Details for CD11b

Target: CD11b (ITGAM) (Integrin alpha M (ITGAM))

Alternative Name: CD11b

Background: CD11b is a cell adhesion molecule that acts as a receptor for cell surface ligands such as intracellular adhesion molecules (ICAMs) or soluble ligands. Integrins are heterodimeric proteins that contain an a chain and b chain. Integrin alpha-M (CD11b) combines with the Integrin beta-2 (CD18) to form a leukocyte-specific integrin referred to as macrophage receptor 1 (Mac-1), or inactivated-C3b (iC3b) receptor 3 (CR3). Integrin alpha-M/beta-2 is important in the adherence of neutrophils and monocytes to stimulated endothelium, and also in the phagocytosis of complement coated particles.

Pathways: Apoptosis, Activation of Innate immune Response, Toll-Like Receptors Cascades, Activated T Cell Proliferation