EpCAM antibody (Cytoplasmic Domain)

Catalog No. ABIN3024723

The Mouse Monoclonal anti-EpCAM antibody is suitable to detect EpCAM in samples from Human, Mouse, Rat, Cat and Dog. It has been validated for IHC (p).

Quick Overview for EpCAM antibody (Cytoplasmic Domain) (ABIN3024723)

Target: EpCAM (EPCAM) (Epithelial Cell Adhesion Molecule (EPCAM))

Reactivity: Human, Mouse, Rat, Cat, Dog

Host: Mouse

Clonality: Monoclonal

Conjugate: This EpCAM antibody is un-conjugated

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: EGP40-1110

Product Details anti-EpCAM Antibody

Binding Specificity: AA 289-314, Cytoplasmic Domain

Purpose: EpCAM Antibody / Cytoplasmic domain

Purification: Protein G affinity chromatography

Immunogen: A portion of amino acids 289-314 (cytoplasmic domain) was used as the immunogen for the EpCAM antibody.

Isotype: IgG2b, kappa

Application Details

Application Notes: Optimal dilution of the EpCAM antibody should be determined by the researcher.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.2 mg/mL

Buffer: 0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store the EpCAM antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Target Details for EpCAM

Target: EpCAM (EPCAM) (Epithelial Cell Adhesion Molecule (EPCAM))

Alternative Name: EpCAM

Background: EGP40 is a 40-43 kDa transmembrane epithelial glycoprotein, also identified as epithelial specific antigen (ESA), or epithelial cellular adhesion molecule (Ep-CAM). It is expressed on baso-lateral cell surface in most simple epithelia and a vast majority of carcinomas. This antibody has been used to distinguish adenocarcinoma from pleural mesothelioma and hepatocellular carcinoma. This antibody is also useful in distinguishing serous carcinomas of the ovary from mesothelioma.

UniProt: P16422