Nkx2-2 antibody

Catalog No. ABIN3024826

The Mouse Monoclonal anti-Nkx2-2 antibody (Clone NX2-294) (ABIN3024826) specifically detects Nkx2-2 in IHC (p). The antibody is reactive with Human and Rat samples.

Quick Overview for Nkx2-2 antibody (ABIN3024826)

Target: Nkx2-2 (NK2 Homeobox 2 (Nkx2-2))

Reactivity: Human, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This Nkx2-2 antibody is un-conjugated

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Grade: Carrier-free

Clone: NX2-294

Product Details anti-Nkx2-2 Antibody

Purpose: NKX2.2 Antibody (azide and preservative free)

Purification: Protein G affinity chromatography

Immunogen: Human NKX2.2 recombinant protein was used as the immunogen for this antibody.

Isotype: IgG2b, kappa

Application Details

Application Notes: The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the NKX2.2 antibody to be titered up or down for optimal performance.

1. Staining of formalin-fixed tissues requires boiling tissue sections in pH 9 10 mM Tris with 1 mM EDTA for 10-20 min followed by cooling at RT for 20 minutes.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: 1 mg/mL in 1X PBS, BSA free, sodium azide free

Preservative: Azide free

Storage: 4 °C,-20 °C

Storage Comment: Store the NKX2.2 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Target Details for Nkx2-2

Target: Nkx2-2 (NK2 Homeobox 2 (Nkx2-2))

Alternative Name: NKX2.2

Background: Expression of NKX2.2 has been found in neuroendocrine tumors of the gut, making it a potential marker for the study of gastrointestinal neuroendocrine tumors. More recently, NKX2.2 protein was identified as a target of EWS-FLI-1, the fusion protein specific to Ewing sarcoma, and was shown to be differentially upregulated in Ewing sarcoma on the basis of array-based gene expression analysis. Antibody to NKX2.2 detects a valuable marker for Ewing sarcoma, with a sensitivity of 93 % and a specificity of 89 %, and aids in the differential diagnosis of small round cell tumors.

Pathways: Dopaminergic Neurogenesis