Mouse anti-Human IgM Antibody

Catalog No. ABIN3024941

Product Details

Target: IgM

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Purification: Protein G

Immunogen: Human Mu heavy chain was used as the immunogen for this IgM antibody.

Clone: MuHC2

Isotype: IgG1 kappa

Application Details

Application Notes: Due to differences in protocols and secondaries, the IgM antibody may need to be titered for optimal performance.

1. FFPE staining requires boiling sections in 10  mM citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. Immunofluorescence: 0.5-1 μg/mL,Western blot: 0.5-1 μg/mL,IHC (FFPE): 0.5-1 μg/mL for 30 minutes at RT (1),Prediluted format : incubate for 30 min at RT (2)

Restrictions: For Research Use only

Handling

Concentration: 0.2 mg/mL

Buffer: 0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store the IgM antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).

Target Details

Target: IgM

Abstract: IgM Products

Target Type: Antibody

Background: IgM is the first antibody generated in an immune response to an antigen. It is generally a pentamer with each of the five immunoglobulins linked together with disulfide bonds. In its pentamer form, it has a molecular mass of 970 kDa and 10 antigen binding sites (due to the large size of most antigens, not all binding sites can be filled simultaneously.  IgM antibodies account for approximately 5 % -10 % of all the antibody in the body.

Gene ID: 3507