NGFR antibody
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- Target See all NGFR Antibodies
- NGFR (Nerve Growth Factor Receptor (NGFR))
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Reactivity
- Human, Non-Human Primate
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Host
- Mouse
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Clonality
- Monoclonal
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Conjugate
- This NGFR antibody is un-conjugated
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Application
- Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- No Cross-Reactivity
- Mouse (Murine), Rat (Rattus)
- Characteristics
- It recognizes a glycoprotein of 75 kDa, identified as low affinity Nerve Growth Factor (NGF) Receptor (p75NGFR) or Neurotrophin Receptor (p75NTR). NGFR is expressed in various neural crest cells and their tumors such as melanocytes, melanomas, neuroblastomas, pheochromocytomas and neurofibromas. Reportedly, anti-NGFR is a reliable marker for desmoplastic and neurotropic melanomas. NGFR is expressed in mature non-neural cells such as perivascular cells, dental pulp cells, lymphoidal follicular dendritic cells, basal epithelium of oral mucosa and hair follicles, prostate basal cells, and myoepithelial cells. Anti-NGFR stains the myoepithelial cells of breast ducts and intra-lobular fibroblasts of breast ducts.
- Purification
- Protein G affinity chromatography
- Immunogen
- Recombinant human protein was used as the immunogen for the NGFR antibody.
- Clone
- NTR-912
- Isotype
- IgG1 kappa
- Top Product
- Discover our top product NGFR Primary Antibody
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- Application Notes
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Optimal dilution of the NGFR antibody should be determined by the researcher.
1. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. Flow Cytometry: 0.5-1 μg/million cells in 0.1ml,Immunofluorescence: 1-2 μg/mL,Immunohistochemistry (FFPE): 0.5-1 μg/mL for 30 min at RT (1),Prediluted format: incubate for 30 min at RT (2) - Restrictions
- For Research Use only
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- Concentration
- 0.2 mg/mL
- Buffer
- 0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05 % sodium azide
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- 4 °C,-20 °C
- Storage Comment
- Store the NGFR antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
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- Target
- NGFR (Nerve Growth Factor Receptor (NGFR))
- Alternative Name
- NGFR (NGFR Products)
- Synonyms
- ngfr antibody, wu:fb38c02 antibody, wu:fc10h01 antibody, CD271 antibody, Gp80-LNGFR antibody, TNFRSF16 antibody, p75(NTR) antibody, p75NTR antibody, LNGFR antibody, RNNGFRR antibody, Tnfrsf16 antibody, p75 antibody, NRH1b antibody, fullback antibody, nrh1 antibody, p75ntr antibody, tnfrsf16 antibody, p75NGFR antibody, nerve growth factor receptor antibody, neurotrophin receptor associated death domain antibody, nerve growth factor receptor S homeolog antibody, nerve growth factor receptor (TNFR superfamily, member 16) antibody, NGFR antibody, nradd antibody, Ngfr antibody, ngfr.S antibody
- Background
- It recognizes a glycoprotein of 75 kDa, identified as low affinity Nerve Growth Factor (NGF) Receptor (p75NGFR) or Neurotrophin Receptor (p75NTR). NGFR is expressed in various neural crest cells and their tumors such as melanocytes, melanomas, neuroblastomas, pheochromocytomas and neurofibromas. Reportedly, anti-NGFR is a reliable marker for desmoplastic and neurotropic melanomas. NGFR is expressed in mature non-neural cells such as perivascular cells, dental pulp cells, lymphoidal follicular dendritic cells, basal epithelium of oral mucosa and hair follicles, prostate basal cells, and myoepithelial cells. Anti-NGFR stains the myoepithelial cells of breast ducts and intra-lobular fibroblasts of breast ducts.
- Pathways
- NF-kappaB Signaling, Neurotrophin Signaling Pathway, Carbohydrate Homeostasis, Growth Factor Binding
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