Prostate Specific Antigen antibody

Catalog No. ABIN3025436

This Mouse Monoclonal antibody specifically detects Prostate Specific Antigen in ELISA, IHC (p), IF and FACS. It exhibits reactivity toward Human.

Quick Overview for Prostate Specific Antigen antibody (ABIN3025436)

Target: Prostate Specific Antigen (PSA)

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Prostate Specific Antigen antibody is un-conjugated

Application: ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF), Flow Cytometry (FACS)

Clone: KLK3-801

Product Details anti-Prostate Specific Antigen Antibody

Purification: Protein G purified

Immunogen: Full length human recombinant protein was used as the immunogen for this PSA antibody.

Isotype: IgG1 kappa

Application Details

Application Notes: Variations in protocols, secondaries and substrates may require the PSA antibody to be titered up or down for optimal performance.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10  mM Citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. ELISA: For coating use Ab at 1-5 μg/mL, order Ab without BSA,FACS: 0.5-1 μg/million cells,IF: 0.5-1 μg/mL,IHC (FFPE): 0.5-1.0 μg/mL for 30 minutes at RT (1),Prediluted format : incubate for 30 min at RT (2)

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: 1 mg/mL in 1X PBS, BSA free, sodium azide free

Preservative: Azide free

Storage: 4 °C,-20 °C

Storage Comment: Store the PSA antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).

Target Details for Prostate Specific Antigen

Target: Prostate Specific Antigen (PSA)

Alternative Name: PSA

Background: Prostate specific antigen / PSA is a chymotrypsin-like serine protease (kallikrein family) exclusively produced by the prostate epithelium, and abundant in seminal fluid. Using a Prostate specific antigen antibody, the protein can be detected in the sera of patients with prostatic carcinoma and expression level correlates positively with the likelyhood of cancer.

Gene ID: 354

Pathways: Complement System