HLA DQ antibody

Catalog No. ABIN3025633

The Mouse Monoclonal anti-HLA DQ antibody (Clone SPV-L3) (ABIN3025633) specifically detects HLA DQ in FACS and IF. The antibody is reactive with Human samples.

Quick Overview for HLA DQ antibody (ABIN3025633)

Target: HLA DQ (HLA-DQ) (MHC Class II HLA-DQ (HLA-DQ))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This HLA DQ antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunofluorescence (IF)

Grade: Carrier-free

Clone: SPV-L3

Product Details anti-HLA DQ Antibody

Purpose: HLA-DQ Antibody (MHC II) (azide and preservative free)

Purification: Protein G affinity chromatography

Immunogen: The T4-positive CTL clone HG-38 was used as the immunogen for the HLA-DQ antibody.

Isotype: IgG2a, kappa

Application Details

Application Notes: Optimal dilution of the HLA-DQ antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: 1 mg/mL in 1X PBS, BSA free, sodium azide free

Preservative: Azide free

Storage: 4 °C,-20 °C

Storage Comment: Store the HLA-DQ antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Target Details for HLA DQ

Target: HLA DQ (HLA-DQ) (MHC Class II HLA-DQ (HLA-DQ))

Alternative Name: HLA-DQ

Background: Recognizes a DQ antigen, which is a dimer of 60 kDa. The class II molecule is a heterodimer consisting of an alpha (DQA) and a beta chain (DQB), both anchored in the membrane. It plays a central role in the immune system by presenting peptides derived from extracellular proteins. Class II molecules are expressed in antigen presenting cells (APC: B Lymphocytes, dendritic cells, macrophages). The alpha chain is approximately 33-35 kDa. It is encoded by 5 exons, exon 1 encodes the leader peptide, exons 2 and 3 encode the two extracellular domains, and exon 4 encodes the transmembrane domain and the cytoplasmic tail. Within the DQ molecule both the alpha chain and the beta chain contain the polymorphisms specifying the peptide binding specificities, resulting in up to four different molecules. Typing for these polymorphisms is routinely done for bone marrow transplantation.

UniProt: P01908