Nuclear Antigen antibody

Catalog No. ABIN3025764

The Mouse Monoclonal anti-Nuclear Antigen antibody is suitable to detect Nuclear Antigen in samples from Human, Mouse and Rat. It has been validated for IHC (p).

Quick Overview for Nuclear Antigen antibody (ABIN3025764)

Target: Nuclear Antigen (IPO-38)

Reactivity: Human, Mouse, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This Nuclear Antigen antibody is un-conjugated

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Grade: Carrier-free

Clone: IPO-38

Product Details anti-Nuclear Antigen Antibody

Purpose: IPO-38 Antibody (Proliferation Marker) (azide and preservative free)

Purification: PEG precipitation

Immunogen: Spleen cells from a patient with hairy cell leukemia were used as the immunogen for the IPO-38 antibody.

Isotype: IgM, kappa

Application Details

Application Notes: Optimal dilution of the IPO-38 antibody should be determined by the researcher.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: 1 mg/mL in 1X PBS, BSA free, sodium azide free

Preservative: Azide free

Storage: 4 °C,-20 °C

Storage Comment: Store the IPO-38 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Target Details for Nuclear Antigen

Target: Nuclear Antigen (IPO-38)

Alternative Name: IPO-38

Background: Recognizes a protein of 14-16 kDa, which is a novel nuclear antigen of proliferating cells. IPO38 antigen is present in the nuclei of proliferating cells such as Hodgkin s disease and non-Hodgkin s lymphomas, different forms of leukemias, breast and colorectal carcinomas, and PHA-stimulated lymphocytes. It is not expressed in the cells of non-stimulated lymphocytes and granulocytes. IPO38 may be a useful marker of cell proliferation during monitoring of tumor progression.