Nuclear Antigen antibody

Catalog No. ABIN7881829

The Mouse Monoclonal anti-Nuclear Antigen antibody is suitable to detect Nuclear Antigen in samples from Human, Mouse and Rat. It has been validated for IHC (p).

Quick Overview for Nuclear Antigen antibody (ABIN7881829)

Target: Nuclear Antigen (IPO-38)

Reactivity: Human, Mouse, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This Nuclear Antigen antibody is un-conjugated

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: SPM515

Product Details anti-Nuclear Antigen Antibody

Purpose: IPO38 Antibody (Proliferation Marker)

Purification: PEG precipitation

Immunogen: Spleen cells from a patient with hairy cell leukemia were used as the immunogen for the IPO38 antibody.

Isotype: IgM, kappa

Application Details

Application Notes: Optimal dilution of the IPO38 antibody should be determined by the researcher.

1. Staining of formalin-fixed tissues requires boiling tissue sections in pH 9 10 mM Tris with 1 mM EDTA for 10-20 min followed by cooling at RT for 20 min.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Prediluted in 1X PBS, 0.1 mg/mL BSA (US sourced), 0.05 % sodium azide, *For IHC use only*

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store the IPO38 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Target Details for Nuclear Antigen

Target: Nuclear Antigen (IPO-38)

Alternative Name: IPO38

Background: Recognizes a protein of 14-16 kDa, which is a novel nuclear antigen of proliferating cells. IPO-38 antigen is present in the nuclei of proliferating cells such as Hodgkin s disease and non-Hodgkin s lymphomas, different forms of leukemias, breast and colorectal carcinomas, and PHA-stimulated lymphocytes. It is not expressed in the cells of non-stimulated lymphocytes and granulocytes. IPO-38 can be a useful marker of cell proliferation during monitoring of tumor progression.