ENO2/NSE antibody (AA 416-433)
-
- Target See all ENO2/NSE (ENO2) Antibodies
- ENO2/NSE (ENO2) (Enolase 2 (Gamma, Neuronal) (ENO2))
-
Binding Specificity
- AA 416-433
-
Reactivity
- Human
-
Host
- Mouse
-
Clonality
- Monoclonal
-
Conjugate
- This ENO2/NSE antibody is un-conjugated
-
Application
- Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- Purification
- Protein G affinity chromatography
- Immunogen
- Amino acids 416-433 of human Neuron-specific Enolase were used as the immunogen for this NSE antibody.
- Clone
- NSEL1-1
- Isotype
- IgG2b kappa
- Top Product
- Discover our top product ENO2 Primary Antibody
-
-
- Application Notes
-
The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the NSE antibody to be titered up or down for optimal performance.
1. Staining of FFPE tissue requires boiling sections in 10 mM Citrate Buffer, pH 6, for 10-20 min followed by cooling at RT for 20 min.\. Western blot: 1-2 μg/mL,FACS: 0.5-1 μg/million cells,IF: 1-2 μg/mL,IHC (FFPE): 0.5-1 μg/mL (1)
- Restrictions
- For Research Use only
-
- Concentration
- 0.2 mg/mL
- Buffer
- 0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05 % sodium azide
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- 4 °C,-20 °C
- Storage Comment
- Store the NSE antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
-
- Target
- ENO2/NSE (ENO2) (Enolase 2 (Gamma, Neuronal) (ENO2))
- Alternative Name
- NSE (ENO2 Products)
- Synonyms
- ENO2 antibody, DKFZp459B1817 antibody, NSE antibody, AI837106 antibody, D6Ertd375e antibody, Eno-2 antibody, RNEN3 antibody, eno3 antibody, wu:fc09h05 antibody, zgc:92418 antibody, enolase 2 antibody, enolase 2 (gamma, neuronal) antibody, enolase 2, gamma neuronal antibody, enolase 2, gamma, neuronal antibody, ENO2 antibody, Eno2 antibody, eno2 antibody
- Background
- Recognizes a protein of about 50 kDa, which is identified as gamma-enolase/neuron specific enolase/enolase 2. Three isoenzymes of enolases are identified, alpha, beta and gamma. Alpha-isoform is expressed in most tissues, whereas beta-form is expressed predominantly in muscle tissue and gamma-enolase is found only in nervous tissue. These isoforms exist as both homodimers and heterodimers, and they play a role in converting phosphoglyceric acid to phosphenolpyruvic acid in the glycolytic pathway. NSE is a useful marker to identify peripheral nerves and tumors of neuro-endocrine origins, such as pheochromocytomas. It it be usually employed in combination with other markers such as Synaptophysin, Chromogranin A, and Neurofilament.
- Gene ID
- 2026
-