FOXA1 antibody (AA 372-472)
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- Target See all FOXA1 Antibodies
- FOXA1 (Forkhead Box A1 (FOXA1))
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Binding Specificity
- AA 372-472
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Reactivity
- Human, Rat
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Host
- Mouse
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Clonality
- Monoclonal
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Conjugate
- This FOXA1 antibody is un-conjugated
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Application
- Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- Purification
- Protein G affinity chromatography
- Immunogen
- A human partial recombinant protein corresponding to amino acids 372-472 was used as the immunogen for this FOXA1 antibody.
- Clone
- FHBA1-1
- Isotype
- IgG
- Top Product
- Discover our top product FOXA1 Primary Antibody
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- Application Notes
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The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the FOXA1 antibody to be titered up or down for optimal performance.
1. Staining of FFPE tissue is enhanced by boiling sections in 10 mM Tris with 1 mM EDTA, pH 9 for 10-20 min followed by cooling at RT for 20 min.\. FACS: 0.5-1 μg/10^6 cells,IF: 1-2 μg/mL,IHC (FFPE): 0.5-1 μg/mL for 30 min at RT (1)
- Restrictions
- For Research Use only
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- Concentration
- 1 mg/mL
- Buffer
- 1 mg/mL in 1X PBS, BSA free, sodium azide free
- Preservative
- Azide free
- Storage
- 4 °C,-20 °C
- Storage Comment
- Store the FOXA1 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
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- Target
- FOXA1 (Forkhead Box A1 (FOXA1))
- Alternative Name
- FOXA1 (FOXA1 Products)
- Synonyms
- HNF3A antibody, TCF3A antibody, Hnf-3a antibody, Hnf3a antibody, Tcf-3a antibody, Tcf3a antibody, fkd7 antibody, forkhead-7 antibody, HNF3-alpha antibody, HNF3alpha antibody, fkh2 antibody, foxa1 antibody, hnf3a antibody, tFoxA1 antibody, tcf3a antibody, xfkh2 antibody, fkh antibody, FOXA1 antibody, Ci-fkh antibody, HNF-3a antibody, forkhead box A1 antibody, forkhead box A1 S homeolog antibody, forkhead homolog antibody, forkhead box A1 L homeolog antibody, FOXA1 antibody, Foxa1 antibody, foxa1 antibody, foxa1.S antibody, fkh antibody, foxa1.L antibody
- Background
- The transcription factor Forkhead-box A1, also known as hepatocyte nuclear factor 3-alpha, is a member of the FOX class of transcription factors. HNF-1 (alpha and beta), HNF-3 (alpha, beta and gamma), HNF-4 (alpha and gamma), and HNF-6 compose, in part, a homeoprotein family designated the hepatocyte nuclear factor family. The various HNF-1 isoforms regulate transcription of genes in the liver as well as in other tissues such as kidney, small intestine and thymus. FOXA1 is expressed in normal breast ductal epithelium and other epithelium in different organs, such as lung, pancreas, bladder, prostate, and colon. Recently, FOXA1 has been shown to be a major determinant of estrogen-ER activity and endocrine response in breast cancer cells. Its expression correlates with estrogen receptor (ER)-positivity, especially in luminal subtype A breast cancers, which is associated with favorable prognosis. FOXA1 is useful in the sub-classification of breast carcinomas.
- Gene ID
- 3169
- Pathways
- Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling, Carbohydrate Homeostasis
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