CD47 antibody

Catalog No. ABIN3026437

This anti-CD47 antibody is a Mouse Monoclonal antibody detecting CD47 in FACS, IF and IHC (p). Suitable for Human and Mouse.

Quick Overview for CD47 antibody (ABIN3026437)

Target: CD47

Reactivity: Human, Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD47 antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: IAP-964

Product Details anti-CD47 Antibody

Characteristics: This antibody reacts with Ig domain of CD47 protein. CD47, originally named integrin-associated protein (IAP), is a 50 kDa protein containing five membrane-spanning sequences and a short cytoplasmic tail. CD47 plays a role in both cell adhesion by acting as an adhesion receptor for THBS1 on platelets, and in the modulation of integrins. It is important in memory formation and synaptic plasticity in the hippocampus. CD47 may play a role in membrane transport and/or integrin dependent signal transduction.

Purification: Protein G affinity chromatography

Immunogen: Recombinant human protein was used as the immunogen for the CD47 antibody.

Isotype: IgG1 kappa

Application Details

Application Notes: Optimal dilution of the CD47 antibody should be determined by the researcher.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10  mM Citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 min.\. Flow Cytometry: 0.5-1 μg/million cells in 0.1ml,Immunofluorescence: 1-2 μg/mL,Immunohistochemistry (FFPE): 0.5-1 μg/mL for 30 min at RT (1)

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: 1 mg/mL in 1X PBS, BSA free, sodium azide free

Preservative: Azide free

Storage: 4 °C,-20 °C

Storage Comment: Store the CD47 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).

Target Details for CD47

Target: CD47

Alternative Name: CD47 (IAP)

Background: This antibody reacts with Ig domain of CD47 protein. CD47, originally named integrin-associated protein (IAP), is a 50  kDa protein containing five membrane-spanning sequences and a short cytoplasmic tail. CD47 plays a role in both cell adhesion by acting as an adhesion receptor for THBS1 on platelets, and in the modulation of integrins. It is important in memory formation and synaptic plasticity in the hippocampus. CD47 may play a role in membrane transport and/or integrin dependent signal transduction.