ErbB2/Her2 antibody

Catalog No. ABIN3026574

The Mouse Monoclonal anti-ErbB2/Her2 antibody is suitable to detect ErbB2/Her2 in samples from Human and Rat. It has been validated for IF and FACS.

Quick Overview for ErbB2/Her2 antibody (ABIN3026574)

Target: ErbB2/Her2 (Receptor tyrosine-protein kinase erbB-2 (ErbB2/Her2))

Reactivity: Human, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This ErbB2/Her2 antibody is un-conjugated

Application: Immunofluorescence (IF), Flow Cytometry (FACS)

Clone: HRB2-258

Product Details anti-ErbB2/Her2 Antibody

Characteristics: This antibody recognizes a protein of 185 kDa, which is identified as HER2 / ErbB2. Its epitope is localized in the extracellular domain. ErbB2 is a member of the EGFR family. This antibody is specific and shows minimal cross-reaction with other members of the EGFR-family. Receptors of this family are located on the plasma membrane and consist of an extracellular ligand-binding domain that is connected to a large intracellular domain by a single transmembrane sequence. ErbB2 protein is over-expressed in a variety of carcinomas especially those of breast and ovary.

Purification: Protein G purified

Immunogen: Recombinant human protein was used as the immunogen for this ErbB2 antibody.(1)

Isotype: IgG2a kappa

Application Details

Application Notes: Variations in protocols, secondaries and substrates may require the antibody to be titered for optimal performance.

1. This ErbB2 antibody binds to the extracellular/cell surface region of the protein.\. FACS: 0.5-1 μg/million cells,IF: 0.5-1 μg/mL

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: 1 mg/mL in 1X PBS, BSA free, sodium azide free

Preservative: Azide free

Storage: 4 °C,-20 °C

Storage Comment: Store the ErbB2 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).

Target Details for ErbB2/Her2

Target: ErbB2/Her2 (Receptor tyrosine-protein kinase erbB-2 (ErbB2/Her2))

Alternative Name: ErbB2 HER2

Background: This antibody recognizes a protein of 185  kDa, which is identified as HER2 / ErbB2. Its epitope is localized in the extracellular domain. ErbB2 is a member of the EGFR family. This antibody is specific and shows minimal cross-reaction with other members of the EGFR-family. Receptors of this family are located on the plasma membrane and consist of an extracellular ligand-binding domain that is connected to a large intracellular domain by a single transmembrane sequence. ErbB2 protein is over-expressed in a variety of carcinomas especially those of breast and ovary.

Pathways: RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Skeletal Muscle Fiber Development