Vimentin antibody

Catalog No. ABIN3026899

The Mouse Monoclonal anti-Vimentin antibody (Clone LN-6) (ABIN3026899) specifically detects Vimentin in IF, FACS and IHC (p). The antibody is reactive with Human, Rat, Mouse, Pig, Cow, Cat, Rabbit and Sheep samples.

Quick Overview for Vimentin antibody (ABIN3026899)

Target: Vimentin (VIM)

Reactivity: Human, Rat, Mouse, Pig, Cow, Cat, Rabbit, Sheep

Host: Mouse

Clonality: Monoclonal

Conjugate: This Vimentin antibody is un-conjugated

Application: Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: LN-6

Product Details anti-Vimentin Antibody

Characteristics: This mAb reacts with a 58 kDa protein identified as vimentin. It reacts with a non-hematopoietic epitope of vimentin and shows no cross-reaction with other closely related intermediate filament proteins (IFP's) such as desmin, keratin, neurofilament, and glial fibrillary acid protein. Vimentin is ubiquitously expressed in mesenchymal cells such as fibroblasts, smooth muscle cells, and endothelium. Antibody against vimentin is useful as part of an antibody panel for differential diagnosis of tumors of unknown origin. Ab-2 does not react with leukocyte common antigen-positive tissues such as lymphomas and leukemias.

Purification: PEG precipitation

Immunogen: A human thymic nuclear extract was used as the immunogen for the Vimentin antibody.

Isotype: IgM kappa

Application Details

Application Notes: Optimal dilution of the Vimentin antibody should be determined by the researcher.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10  mM Citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 min.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. Flow Cytometry: 0.5-1 μg/million cells in 0.1ml,Immunofluorescence: 0.5-1 μg/mL,Immunohistochemistry (FFPE): 1-2 μg/mL for 30 min at RT (1),Prediluted format: incubate for 30 min at RT (2)

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: 1 mg/mL in 1X PBS, BSA free, sodium azide free

Preservative: Azide free

Storage: 4 °C,-20 °C

Storage Comment: Store the Vimentin antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).

Target Details for Vimentin

Target: Vimentin (VIM)

Alternative Name: Vimentin

Background: This mAb reacts with a 58  kDa protein identified as vimentin. It reacts with a non-hematopoietic epitope of vimentin and shows no cross-reaction with other closely related intermediate filament proteins (IFP's) such as desmin, keratin, neurofilament, and glial fibrillary acid protein. Vimentin is ubiquitously expressed in mesenchymal cells such as fibroblasts, smooth muscle cells, and endothelium. Antibody against vimentin is useful as part of an antibody panel for differential diagnosis of tumors of unknown origin. Ab-2 does not react with leukocyte common antigen-positive tissues such as lymphomas and leukemias.

Pathways: Caspase Cascade in Apoptosis