Vimentin antibody

Catalog No. ABIN3026899

The Mouse Monoclonal anti-Vimentin antibody (Clone LN-6) (ABIN3026899) specifically detects Vimentin in IHC (p). The antibody is reactive with Human, Rat and Mouse samples.

Quick Overview for Vimentin antibody (ABIN3026899)

Target: Vimentin (VIM)

Reactivity: Human, Rat, Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This Vimentin antibody is un-conjugated

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Grade: Carrier-free

Clone: LN-6

Product Details anti-Vimentin Antibody

Purpose: Vimentin Antibody (azide and preservative free)

Characteristics: This mAb reacts with a 58 kDa protein identified as vimentin. It reacts with a non-hematopoietic epitope of vimentin and shows no cross-reaction with other closely related intermediate filament proteins (IFP's) such as desmin, keratin, neurofilament, and glial fibrillary acid protein. Vimentin is ubiquitously expressed in mesenchymal cells such as fibroblasts, smooth muscle cells, and endothelium. Antibody against vimentin is useful as part of an antibody panel for differential diagnosis of tumors of unknown origin. Ab-2 does not react with leukocyte common antigen-positive tissues such as lymphomas and leukemias.

Purification: PEG precipitation

Immunogen: A human thymic nuclear extract was used as the immunogen for the Vimentin antibody.

Isotype: IgM, kappa

Application Details

Application Notes: Optimal dilution of the Vimentin antibody should be determined by the researcher.

1. Staining of formalin-fixed tissues requires boiling tissue sections in pH 9 10 mM Tris with 1 mM EDTA for 10-20 min followed by cooling at RT for 20 min.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: 1 mg/mL in 1X PBS, BSA free, sodium azide free

Preservative: Azide free

Storage: 4 °C,-20 °C

Storage Comment: Store the Vimentin antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Target Details for Vimentin

Target: Vimentin (VIM)

Alternative Name: Vimentin

Background: This mAb reacts with a 58 kDa protein identified as vimentin. It reacts with a non-hematopoietic epitope of vimentin and shows no cross-reaction with other closely related intermediate filament proteins (IFP's) such as desmin, keratin, neurofilament, and glial fibrillary acid protein. Vimentin is ubiquitously expressed in mesenchymal cells such as fibroblasts, smooth muscle cells, and endothelium. Antibody against vimentin is useful as part of an antibody panel for differential diagnosis of tumors of unknown origin. Ab-2 does not react with leukocyte common antigen-positive tissues such as lymphomas and leukemias.

UniProt: P08670

Pathways: Caspase Cascade in Apoptosis