VDAC1 antibody (Middle Region)

Catalog No. ABIN3029602

Product Details anti-VDAC1 Antibody

Target: VDAC1 (Voltage-Dependent Anion Channel 1 (VDAC1))

Binding Specificity: Middle Region

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This VDAC1 antibody is un-conjugated

Application: Western Blotting (WB), Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Purification: Antigen affinity

Immunogen: An amino acid sequence from the middle region of human Voltage-dependent anion channel 1 (RVTQSNFAVGYKTDEF) was used as the immunogen for this VDAC1 antibody (100% homologous in human, mouse and rat).

Isotype: IgG

Application Details

Application Notes: The stated application concentrations are suggested starting amounts. Titration of the VDAC1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Western blot: 0.5-1 μg/mL,IHC (Paraffin): 0.5-1 μg/mL,IHC (Frozen): 0.5-1 μg/mL,Immunocytochemistry: 0.5-1 μg/mL

Restrictions: For Research Use only

Handling

Buffer: 0.5 mg/mL if reconstituted with 0.2 mL sterile DI water

Storage: -20 °C

Storage Comment: After reconstitution, the VDAC1 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Target Details for VDAC1

Target: VDAC1 (Voltage-Dependent Anion Channel 1 (VDAC1))

Alternative Name: VDAC1 (VDAC1 Products)

Synonyms: vdac1 antibody, VDAC1 antibody, 5076 antibody, ARABIDOPSIS THALIANA VOLTAGE DEPENDENT ANION CHANNEL 1 antibody, ATVDAC1 antibody, T22N4.9 antibody, T22N4_9 antibody, voltage dependent anion channel 1 antibody, PORIN antibody, VDAC-1 antibody, AL033343 antibody, Vdac5 antibody, fa13f11 antibody, wu:fa13f11 antibody, zgc:85830 antibody, voltage-dependent anion channel 1 S homeolog antibody, voltage-dependent anion channel 1 antibody, voltage dependent anion channel 1 antibody, vdac1.S antibody, vdac1 antibody, VDAC1 antibody, Vdac1 antibody

Background: The voltage-dependent anion channel of the outer mitochondrial membrane is a small, abundant outer membrane pore-forming protein found in the outer membranes of all eukaryotic mitochondria. The protein is though to form the major pathway for movement of adenine nucleotides through the outer membrane and to be the mitochondrial binding site for hexokinase and glycerol kinase. At low transmembrane voltage, VDAC1 is open for anions such as phosphate, chloride, and adenine nucleotides. At higher transmembrane voltage, VDAC1 functions as a selective channel for cations and uncharged molecules. These features make it likely the protein plays a role in mitochondrial energy metabolism. Huizing et al. studied by Northern and Western blot analyses the human tissue distribution of mitochondrial transmembrane metabolite carriers. VDAC1 mRNA has a ubiquitous distribution, with most pronounced expression in heart, liver, and skeletal muscle, whereas the VDAC2 isoform appears to be expressed only in the heart.

UniProt: P21796