BAX antibody (AA 19-33)

Catalog No. ABIN3030143

The Rabbit Polyclonal anti-BAX antibody is suitable to detect BAX in samples from Human, Mouse and Rat. It has been validated for WB and FACS.

Quick Overview for BAX antibody (AA 19-33) (ABIN3030143)

Target: BAX (BCL2-Associated X Protein (BAX))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This BAX antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS)

Product Details anti-BAX Antibody

Binding Specificity: AA 19-33

Purification: Antigen affinity

Immunogen: Amino acids 19-33 (IMKTGALLLQGFIQD) were used as the immunogen for this Bax antibody.

Isotype: IgG

Application Details

Application Notes: The stated application concentrations are suggested starting points. Titration of the Bax antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Western blot: 0.5-1 μg/mL,FACS: 1-3 μg/10^6 cells

Restrictions: For Research Use only

Handling

Buffer: 0.5 mg/mL if reconstituted with 0.2 mL sterile DI water

Storage: -20 °C

Storage Comment: After reconstitution, the Bax antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Target Details for BAX

Target: BAX (BCL2-Associated X Protein (BAX))

Alternative Name: Bax

Background: Bcl-2 Associated X Protein is a member of the Bcl-2 gene family. The gene encodes a 21- kDa protein whose association with Bcl-2 is believed to play a critical role in regulating apoptosis. It is an apoptosis-inducing protein that participates in cell death during normal development and in various diseases. It resides in an inactive state in the cytosol of many cells. Bax consists of 9 alpha helices and has extensive amino acid homology with Bcl-2, focused within highly conserved domains I and II. The protein is encoded by six exons and demonstrates a complex pattern of alternative RNA splicing that predicts a 21 kd membrane (alpha) and two forms of cytosolic protein (beta and gamma). Bax and Bak are essential for regulating the number of B cells at both immature and mature developmental stages. They are critical mediators of B cell death induced by multiple stimuli.

UniProt: Q07812

Pathways: p53 Signaling, PI3K-Akt Signaling, Apoptosis, Caspase Cascade in Apoptosis, Positive Regulation of Endopeptidase Activity, Unfolded Protein Response