Aurora A antibody

Catalog No. ABIN303027

The Goat Polyclonal anti-Aurora A antibody (ABIN303027) specifically detects Aurora A in WB, IHC (p) and EIA. The antibody is reactive with Human samples.

Quick Overview for Aurora A antibody (ABIN303027)

Target: Aurora A (AURKA) (Aurora Kinase A (AURKA))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This Aurora A antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Product Details anti-Aurora A Antibody

Specificity: This antibody reacts to the C Terminus of Aurora kinase A / STK15: peptide sequence (QNKESASKQS).

Purification: Immunoaffinity Chromatography

Immunogen: Synthetic peptide

Application Details

Application Notes: Immunohistochemistry on paraffin sectons: 10 μg/mL. Western Blot: 0,5 - 2 μg/mL. ELISA.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris buffered saline containing 0.5 % BSA and 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C

Target Details for Aurora A

Target: Aurora A (AURKA) (Aurora Kinase A (AURKA))

Alternative Name: Aurora Kinase A

Background: ARK1, an Aurora/IPL1-like protein kinase, functions in centrosome separation, duplication, and maturation and in bipolar spindle assembly and stability. The protein has been shown to localize to centrosomes in S phase as soon as centrioles have been duplicated, and to be degraded in early G1. ARK1 phosphorylation is induced by okadaic acid treatment in vivo. Activated ARK1 has been shown to phosphorylate the spindle apparatus-associated protein, TPX2, and it is believed to be targeted to the spindle apparatus by this protein. At least three alternative splice forms have been reported, however, the alternative splice sites are located in the 5'-untranslated region and appear to have no effect on protein translation. ARK1 has been identified as an oncogene that is overexpressed in several types of solid tumors and tumor cell lines, including those from breast and colon. Additionally, overexpression of ARK1 has been implicated in mechanisms leading to mitotic spindle aberrations, aneuploidy, and genomic instability.Synonyms: AIK1, ARK1, AURA, AURKA, AURORA2, Aurora/IPL1-related kinase 1, BTAK, Breast tumor-amplified kinase, STK15, STK6, Serine/threonine kinase 15, Serine/threonine-protein kinase 6

Gene ID: 6790

NCBI Accession: NP_003591

UniProt: O14965

Pathways: Cell Division Cycle, Asymmetric Protein Localization