Caspase 1 antibody (N-Term, Subunit p20)

Catalog No. ABIN3030287

The Rabbit Polyclonal anti-Caspase 1 antibody is suitable to detect Caspase 1 in samples from Mouse and Rat. It has been validated for WB.

Quick Overview for Caspase 1 antibody (N-Term, Subunit p20) (ABIN3030287)

Target: Caspase 1 (CASP1)

Reactivity: Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Caspase 1 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-Caspase 1 Antibody

Binding Specificity: N-Term, Subunit p20

Purpose: Caspase-1 Antibody (p20 subunit)

Sequence: LEKAQKLWKE NPSEIYPIMN T

Purification: Antigen affinity

Immunogen: An amino acid sequence from the N-terminus of mouse CASP1 (LEKAQKLWKENPSEIYPIMNT) was used as the immunogen for this Caspase-1 antibody. This sequence is from the p20 subunit.

Isotype: IgG

Application Details

Application Notes: The stated application concentrations are suggested starting points. Titration of the Caspase-1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: 0.5 mg/mL if reconstituted with 0.2 mL sterile DI water

Storage: 4 °C,-20 °C

Storage Comment: After reconstitution, the Caspase-1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Target Details for Caspase 1

Target: Caspase 1 (CASP1)

Alternative Name: Caspase-1

Background: Caspase-1 is a cysteine protease that regulates inflammatory processes through its capacity to process and activate the interleukin-1-beta, IL-18, and IL-33 precursor proteins. It belongs to a family of cysteine proteases known as caspases that always cleave proteins following an aspartic acid residue. The Caspase-1 gene consists of 10 exons spanning at least 10.6 kb and is mapped to 11q23, a site frequently involved in rearrangement in human cancers, including a number of leukemias and lymphomas, by Southern DNA blot analysis of rodent-human hybrids and by in situ hybridization to normal human metaphase chromosomes. Caspase 1 has been shown to induce cell necrosis or pyroptosis and may function in various developmental stages.

UniProt: P29452

Pathways: Apoptosis, Interferon-gamma Pathway, Positive Regulation of Endopeptidase Activity, Inflammasome