Caspase 8 antibody (AA 240-259)

Catalog No. ABIN3030316

This Rabbit Polyclonal antibody specifically detects Caspase 8 in WB. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for Caspase 8 antibody (AA 240-259) (ABIN3030316)

Target: Caspase 8 (CASP8)

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Caspase 8 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-Caspase 8 Antibody

Binding Specificity: AA 240-259

Purification: Antigen affinity

Immunogen: Amino acids 240-259 (DKVYQMKSKPRGYCLIINNH-human) were used as the immunogen for this anti-Caspase-8 antibody. This sequence is from the large subunit.

Isotype: IgG

Application Details

Application Notes: The stated application concentrations are suggested starting points. Titration of the anti-Caspase-8 antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Western blot: 0.5-1 μg/mL

Restrictions: For Research Use only

Handling

Buffer: 0.5 mg/mL if reconstituted with 0.2 mL sterile DI water

Storage: -20 °C

Storage Comment: After reconstitution, the anti-Caspase-8 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Target Details for Caspase 8

Target: Caspase 8 (CASP8)

Alternative Name: Caspase-8

Background: Most upstream protease of the activation cascade of caspases responsible for the TNFRSF6/FAS mediated and TNFRSF1A induced cell death. Binding to the adapter molecule FADD recruits it to either receptor. The resulting aggregate called death-inducing signaling complex (DISC) performs CASP8 proteolytic activation. The active dimeric enzyme is then liberated from the DISC and free to activate downstream apoptotic proteases including CASP3/4/6/7/9/10. [UniProt]

UniProt: Q14790

Pathways: Apoptosis, Caspase Cascade in Apoptosis, TLR Signaling, Activation of Innate immune Response, Tube Formation, Positive Regulation of Endopeptidase Activity, Toll-Like Receptors Cascades