CD68 antibody (AA 312-326)

Catalog No. ABIN3030412

Product Details anti-CD68 Antibody

Target: CD68 (CD68 Molecule (CD68))

Binding Specificity: AA 312-326

Reactivity: Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CD68 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Purification: Antigen affinity

Immunogen: Amino acids 312-326 (AFCITRRRQSTYQPL-mouse) were used as the immunogen for this CD68 antibody.

Isotype: IgG

Application Details

Application Notes: The stated application concentrations are suggested starting amounts. Titration of the CD68 antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Western blot: 0.5-1 μg/mL,IHC (Paraffin): 0.5-1 μg/mL,IHC (Frozen): 0.5-1 μg/mL

Restrictions: For Research Use only

Handling

Buffer: 0.5 mg/mL if reconstituted with 0.2 mL sterile DI water

Storage: -20 °C

Storage Comment: After reconstitution, the CD68 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

References for anti-CD68 antibody (ABIN3030412)

Minasyan, Sharma, Pivarnik, Liu, Adams, Bermejo, Peng, Liu, Ishikawa, Perry, Kaminski, Gulati, Herzog, Dela Cruz, Ryu: "Elevated IL-15 concentrations in the sarcoidosis lung is independent of granuloma burden and disease phenotypes." in: American journal of physiology. Lung cellular and molecular physiology, (2021) (PubMed).

Han, Qian, Jiang, Figetakis, Kosyakova, Tellides, Niklason, Chang: "A therapeutic vascular conduit to support in vivo cell-secreted therapy." in: NPJ Regenerative medicine, Vol. 6, Issue 1, pp. 40, (2021) (PubMed).

Target Details for CD68

Target: CD68 (CD68 Molecule (CD68))

Alternative Name: CD68 (CD68 Products)

Synonyms: Lamp4 antibody, Scard1 antibody, gp110 antibody, GP110 antibody, LAMP4 antibody, SCARD1 antibody, CD68 molecule antibody, CD68 antigen antibody, Cd68 molecule antibody, CD68 antibody, Cd68 antibody

Background: Cluster of differentiation 68 is a transmembrane glycoprotein that is highly expressed by human monocytes and tissue macrophages. CD68 is a member of a family of hematopoietic mucin-like molecules that includes leukosialin/CD43 and stem cell antigen CD34. The gene is mapped to 17p13.1. Immunohistochemistry can be used to identify the presence of CD68, which is found in the cytoplasmic granules of a range of different blood cells. It is particularly useful as a marker for the various cells of the macrophage lineage, including monocytes, histiocytes, giant cells, Kupffer cells, and osteoclasts. This allows it to be used to distinguish diseases of otherwise similar appearance, such as the monocyte/macrophage and lymphoid forms of leukaemia(the latter being CD68 negative). Its presence in macrophages also makes it useful in diagnosing conditions related to proliferation or abnormality of these cells, such as malignant histiocytosis, histiocytic lymphoma, and Gaucher's disease.

UniProt: P31996