EIF2AK2 antibody (N-Term)

Catalog No. ABIN3030883

This Rabbit Polyclonal antibody specifically detects EIF2AK2 in WB. It exhibits reactivity toward Human.

Quick Overview for EIF2AK2 antibody (N-Term) (ABIN3030883)

Target: EIF2AK2 (Eukaryotic Translation Initiation Factor 2-alpha Kinase 2 (EIF2AK2))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This EIF2AK2 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-EIF2AK2 Antibody

Binding Specificity: N-Term

Purification: Antigen affinity

Immunogen: An amino acid sequence from the N-terminus of human Protein kinase RNA activated (AGDLSAGFFMEELNTYR) was used as the immunogen for this PKR antibody.

Isotype: IgG

Application Details

Application Notes: The stated application concentrations are suggested starting amounts. Titration of the PKR antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Western blot: 0.5-1 μg/mL

Restrictions: For Research Use only

Handling

Buffer: 0.5 mg/mL if reconstituted with 0.2 mL sterile DI water

Storage: -20 °C

Storage Comment: After reconstitution, the PKR antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Target Details for EIF2AK2

Target: EIF2AK2 (Eukaryotic Translation Initiation Factor 2-alpha Kinase 2 (EIF2AK2))

Alternative Name: PKR

Background: Eukaryotic Translation Initiation Factor 2-Alpha Kinase 2, also called PKR (Protein kinase RNA activated), is an enzyme that in humans is encoded by the EIF2AK2 gene. Activation of the protein allows the kinase to phosphorylate its natural substrate, the alpha subunit of eukaryotic protein synthesis initiation factor-2, leading to the inhibition of protein synthesis(summary by Kuhen et al., 1996). By FISH analysis, Squire et al.(1993) assigned the gene to the boundary between chromosome 2p22-p21. Ben-Asouli et al.(2002) showed that human gamma-interferon mRNA uses local activation of PKR in the cell to control its own translation yield. IFNG mRNA was found to activate PKR through a pseudoknot in its 5-prime untranslated region. Taylor et al.(1999) studied the mechanism underlying the resistance of hepatitis C virus(HCV) to interferon. They demonstrated that the HCV envelope protein E2 contains a sequence identical with phosphorylation sites of the interferon-inducible protein kinase PKR and its target, the translation initiation factor EIF2-alpha. E2 inhibited the kinase activity of the protein and blocked its inhibitory effect on protein synthesis and cell growth.

UniProt: P19525

Pathways: DNA Damage Repair, ER-Nucleus Signaling, Hepatitis C