Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
Purification
Antigen affinity
Immunogen
An amino acid sequence from the N-terminus of human Flavin containing monooxygenase 4 (HEDYPNFMNHEKFWDYLQ) was used as the immunogen for this FMO4 antibody.
FMO4
Reactivity: Human
ELISA
Host: Rabbit
Polyclonal
Biotin
Application Notes
The stated application concentrations are suggested starting amounts. Titration of the FMO4 antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Western blot: 0.5-1 μg/mL,IHC (Paraffin): 0.5-1 μg/mL
Restrictions
For Research Use only
Buffer
0.5 mg/mL if reconstituted with 0.2 mL sterile DI water
Storage
-20 °C
Storage Comment
After reconstitution, the FMO4 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
Flavin-containing monooxygenase 4 (formerly FMO2) is an enzyme that in humans is encoded by the FMO4 gene. By PCR analysis, Dolphin et al.(1992) mapped the gene to chromosome 1. Southern blot hybridization with single exon probes demonstrated that human FMO4 and FMO1 are, in each case, the product of a single gene. Lawton et al.(1994) proposed a reclassification of mammalian FMOs. Under this system, FMO2 described by Dolphin et al.(1992) is now designated FMO4.