GPER antibody (C-Term)

Catalog No. ABIN3031013

The Rabbit Polyclonal anti-GPER antibody has been validated for WB. It is suitable to detect GPER in samples from Human, Mouse, Rat and Monkey.

Quick Overview for GPER antibody (C-Term) (ABIN3031013)

Target: GPER (G Protein-Coupled Estrogen Receptor 1 (GPER))

Reactivity: Human, Mouse, Rat, Monkey

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GPER antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-GPER Antibody

Binding Specificity: C-Term

Purification: Antigen affinity

Immunogen: An amino acid sequence from the C-terminus of human G-protein coupled receptor 30 (ENVFISVHLLQRTQ) was used as the immunogen for this GPR30 antibody.

Isotype: IgG

Application Details

Application Notes: The stated application concentrations are suggested starting amounts. Titration of the GPR30 antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Western blot: 0.5-1 μg/mL

Restrictions: For Research Use only

Handling

Buffer: 0.5 mg/mL if reconstituted with 0.2 mL sterile DI water

Storage: -20 °C

Storage Comment: After reconstitution, the GPR30 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Target Details for GPER

Target: GPER (G Protein-Coupled Estrogen Receptor 1 (GPER))

Alternative Name: GPR30

Background: G-protein coupled estrogen receptor 1 (GPER1), also known as the GPR30 or CEPR, is a G protein-coupled receptor that in humans is encode d by the GPER gene. This gene is a member of the G-protein coupled receptor 1 family and encodes a multi-pass membrane protein that localizes to the endoplasmic reticulum. The protein binds estrogen, resulting in intracellular calcium mobilization and synthesis of phosphatidylinositol 3,4,5-trisphosphate in the nucleus. GPR30 therefore plays a role in the rapid nongenomic signaling events widely observed following stimulation of cells and tissues with estrogen. Alternate transcriptional splice variants which encode the same protein have been characterized.

UniProt: Q99527

Pathways: EGFR Signaling Pathway, Positive Regulation of Peptide Hormone Secretion, Intracellular Steroid Hormone Receptor Signaling Pathway, Steroid Hormone Mediated Signaling Pathway, Carbohydrate Homeostasis, cAMP Metabolic Process, Regulation of G-Protein Coupled Receptor Protein Signaling, Positive Regulation of Endopeptidase Activity, Regulation of Carbohydrate Metabolic Process