HSD17B1 antibody (AA 29-43)

Catalog No. ABIN3031269

This anti-HSD17B1 antibody is a Rabbit Polyclonal antibody detecting HSD17B1 in WB, ICC and IHC (p). Suitable for Human, Rat and Mouse.

Quick Overview for HSD17B1 antibody (AA 29-43) (ABIN3031269)

Target: HSD17B1 (Hydroxysteroid (17-Beta) Dehydrogenase 1 (HSD17B1))

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HSD17B1 antibody is un-conjugated

Application: Western Blotting (WB), Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-HSD17B1 Antibody

Binding Specificity: AA 29-43

Purification: Antigen affinity

Immunogen: Amino acids 29-43 (QSFKVYATLRDLKTQ-human) were used as the immunogen for this HSD17B1 antibody.

Isotype: IgG

Application Details

Application Notes: The stated application concentrations are suggested starting amounts. Titration of the HSD17B1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Western blot: 0.5-1 μg/mL,IHC (Paraffin): 0.5-1 μg/mL,Immunocytochemistry: 0.5-1 μg/mL

Restrictions: For Research Use only

Handling

Buffer: 0.5 mg/mL if reconstituted with 0.2 mL sterile DI water

Storage: -20 °C

Storage Comment: After reconstitution, the HSD17B1 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Target Details for HSD17B1

Target: HSD17B1 (Hydroxysteroid (17-Beta) Dehydrogenase 1 (HSD17B1))

Alternative Name: HSD17B1

Background: 17-beta-Hydroxysteroid dehydrogenas I, also known as 17-beta-hydroxysteroid dehydrogenase or 17-ketosteroid reductase, is an enzyme that is responsible for the interconversion of estrone(E1) and estradiol(E2) as well as the interconversion of androstenedione and testosterone. HSD17B1 gene expression is detected in 34 of 42 (81 % ) adenomas in all tumor subtypes, HSD17B2 mRNA is detected in 18 of 42(43 %) adenomas but not in prolactinomas. Human estrogenic HSD17B1 is an NADP(H)-preferring enzyme. It possesses 11- and 4-fold higher specificity toward NADP(H) over NAD(H) for oxidation and reduction, respectively, as demonstrated by kinetic studies. All four isoforms are variably expressed in human anterior pituitary adenomas, which also show HSD17B enzyme activity, suggesting that it may play an important role in regulating the local cellular levels of estradiol.

UniProt: P14061

Pathways: Metabolism of Steroid Hormones and Vitamin D, Steroid Hormone Biosynthesis