KLF5 antibody (Middle Region)

Catalog No. ABIN3031574

Product Details anti-KLF5 Antibody

Target: KLF5 (Kruppel-Like Factor 5 (Intestinal) (KLF5))

Binding Specificity: Middle Region

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This KLF5 antibody is un-conjugated

Application: Western Blotting (WB)

Purification: Antigen affinity

Immunogen: An amino acid sequence from the middle region of human Kruppel-like factor 5 (HKKYRRDSASVVDQFFTD) was used as the immunogen for this KLF5 antibody (100% homologous in human, mouse and rat).

Isotype: IgG

Application Details

Application Notes: The stated application concentrations are suggested starting amounts. Titration of the KLF5 antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Western blot: 0.5-1 μg/mL

Restrictions: For Research Use only

Handling

Buffer: 0.5 mg/mL if reconstituted with 0.2 mL sterile DI water

Storage: -20 °C

Storage Comment: After reconstitution, the KLF5 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Target Details for KLF5

Target: KLF5 (Kruppel-Like Factor 5 (Intestinal) (KLF5))

Alternative Name: KLF5 (KLF5 Products)

Synonyms: KLF5 antibody, klf5 antibody, MGC115081 antibody, BTEB2 antibody, Bteb2 antibody, IKLF antibody, bteb2 antibody, 4930520J07Rik antibody, CKLF antibody, Kruppel like factor 5 antibody, Kruppel-like factor 5 (intestinal) S homeolog antibody, Kruppel-like factor 5 antibody, KLF5 antibody, klf5.S antibody, Klf5 antibody

Background: Kruppel-like factor 5 is a protein that in humans is encoded by the KLF5 gene. It is also known as BTEB2. This gene encodes a member of the Kruppel-like factor subfamily of zinc finger proteins. Suske et al.(2005) stated that the human gene maps to chromosome 13q21.33, and the mouse gene to chromosome 14E2.1. Sogawa et al.(1993) isolated a cDNA encoding KLF5. The predicted protein contains 3 consecutive zinc finger motifs near the C terminus. The zinc finger domains were 59 % and 64 % identical to those of Sp1 (189906) and BTEB1, respectively. Both KLF5 and Sp1 have a short basic region preceding the zinc finger motifs that may play an auxiliary role in DNA binding. The N-terminal region of KLF5 is proline rich. Recombinant KLF5 protein and Sp1 showed very similar DNA-binding specificity in vitro. When expressed in mammalian cells, KLF5 activated the transcription of a reporter gene fused to a GC box-containing promoter. Sogawa et al.(1993) also identified the rat homolog.

UniProt: Q13887