MBD4 antibody (C-Term)

Catalog No. ABIN3031772

The Rabbit Polyclonal anti-MBD4 antibody is suitable to detect MBD4 in samples from Human, Mouse and Rat. It has been validated for WB.

Quick Overview for MBD4 antibody (C-Term) (ABIN3031772)

Target: MBD4 (Methyl-CpG Binding Domain Protein 4 (MBD4))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MBD4 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-MBD4 Antibody

Binding Specificity: C-Term

Purpose: MBD4 Antibody

Sequence: YHDWLWENHE KLSLS

Purification: Antigen affinity

Immunogen: An amino acid sequence from the C-terminus of human Methyl-CpG-Binding Domain Protein 4 (YHDWLWENHEKLSLS) was used as the immunogen for this MBD4 antibody (100% homologous in human, mouse and rat).

Isotype: IgG

Application Details

Application Notes: The stated application concentrations are suggested starting amounts. Titration of the MBD4 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: 0.5 mg/mL if reconstituted with 0.2 mL sterile DI water

Storage: 4 °C,-20 °C

Storage Comment: After reconstitution, the MBD4 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Target Details for MBD4

Target: MBD4 (Methyl-CpG Binding Domain Protein 4 (MBD4))

Alternative Name: MBD4

Background: Methyl-CpG-Binding Domain Protein 4, also known as MED1, is a protein that in humans is encoded by the MBD4 gene. MBD4 specifically binds methylated DNA, colocalizes with methylated sequences, and is likely to mediate the effects of DNA methylation in mammalian cells(Hendrich and Bird, 1998). Riccio et al.(1999) mapped the gene to chromosome 3q21-q22 by FISH. Hendrich and Bird(1998) found that both MBD2 and -4 specifically bound methylated DNA in vitro and colocalized with methylated sequences in vivo. They concluded that MBD2 and -4 are likely to be mediators of the effects of DNA methylation in mammalian cells. Hendrich et al.(1999) showed that MBD4 contains a methyl-CpG-binding domain that can efficiently remove thymine or uracil from mismatched CpG sites in vitro. Furthermore, the methyl-CpG-binding domain of MBD4 binds preferentially to 5-methylcytosine CpG-TpG mismatches--the primary product of deamination at methyl-CpG.

UniProt: O95243

Pathways: DNA Damage Repair