PLA2G4A antibody (C-Term)

Catalog No. ABIN3032229

Product Details anti-PLA2G4A Antibody

Target: PLA2G4A (Phospholipase A2, Group IVA (Cytosolic, Calcium-Dependent) (PLA2G4A))

Binding Specificity: C-Term

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PLA2G4A antibody is un-conjugated

Application: Western Blotting (WB)

Purification: Antigen affinity

Immunogen: An amino acid sequence from the C-terminus of human Phospholipase A2 (PNQAFKRLHDLMHFN) was used as the immunogen for this CPLA2 antibody.

Isotype: IgG

Application Details

Application Notes: The stated application concentrations are suggested starting amounts. Titration of the CPLA2 antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Western blot: 0.5-1 μg/mL

Restrictions: For Research Use only

Handling

Buffer: 0.5 mg/mL if reconstituted with 0.2 mL sterile DI water

Storage: -20 °C

Storage Comment: After reconstitution, the CPLA2 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Target Details for PLA2G4A

Target: PLA2G4A (Phospholipase A2, Group IVA (Cytosolic, Calcium-Dependent) (PLA2G4A))

Alternative Name: CPLA2 (PLA2G4A Products)

Synonyms: PLA2G4 antibody, cPLA2-alpha antibody, Pla2c antibody, Pla2g4 antibody, cPLA2 antibody, pla2g4 antibody, CPLA2 antibody, PLA2G4A antibody, PLA2 antibody, PLA2G1B antibody, cPLA2alpha antibody, pla2g4a antibody, si:dkey-97o5.1 antibody, phospholipase A2 group IVA antibody, phospholipase A2 group IVA L homeolog antibody, phospholipase A2, group IVA (cytosolic, calcium-dependent) antibody, phospholipase A2, group IVAb (cytosolic, calcium-dependent) antibody, PLA2G4A antibody, Pla2g4a antibody, pla2g4a.L antibody, pla2g4ab antibody

Background: Cytosolic Phospholipase A2, Group IVA is an enzyme that in humans is encoded by the PLA2G4A gene. Tay et al.(1995) mapped the gene to rat chromosome 13 by PCR-based intercross genotyping and to human 1q25 by fluorescence in situ hybridization. By site-directed mutagenesis and biochemical analysis of the recombinant protein, Sharp et al.(1994) determined that ser228 participates in the catalytic mechanism of cPLA2 and that both the phospholipase A2 and the lysophospholipase activities are catalyzed by the same active site residue(s). PLA2G4A, the cytosolic phospholipase A2, appears to subserve transmembrane signaling responses to extracellular ligands(Skorecki, 1995).

UniProt: P47712

Pathways: Inositol Metabolic Process, G-protein mediated Events, VEGF Signaling