c-MET antibody (AA 208-407)

Catalog No. ABIN3042360

This anti-c-MET antibody is a Rabbit Polyclonal antibody detecting c-MET in WB. Suitable for Human and Rat. This Primary Antibody has been cited in 3+ publications.

Quick Overview for c-MET antibody (AA 208-407) (ABIN3042360)

Target: c-MET (MET) (Met Proto-Oncogene (MET))

Reactivity: Human, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This c-MET antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-c-MET Antibody

Binding Specificity: AA 208-407

Purpose: Anti-Met (c-Met) Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins

Characteristics: Anti-Met (c-Met) Antibody Picoband® (ABIN3042360). Tested in WB applications. This antibody reacts with Human, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human Met recombinant protein (Position: D208-S407). Human Met shares 90% and 91% amino acid (aa) sequences identity with mouse and rat Met, respectively.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL, Rat, Human
1. Bottaro DP, Rubin JS, Faletto DL, Chan AM, Kmiecik TE, Vande Woude GF, Aaronson SA (February 1991). "Identification of the hepatocyte growth factor receptor as the met proto-oncogene product". Science 251 (4995): 802-4. 2. Galland F, Stefanova M, Lafage M, Birnbaum D (1992). "Localization of the 5' end of the MCF2 oncogene to human chromosome 15q15----q23". Cytogenet. Cell Genet. 60 (2): 114-6. 3. Cooper CS (January 1992). "The met oncogene: from detection by transfection to transmembrane receptor for hepatocyte growth factor". Oncogene 7 (1): 3-7.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

References for anti-c-MET antibody (ABIN3042360)

Chen, Ding, Gao, Sun, Zhang, Du, Qiu, Wang, Zheng, Sun, Ni, Feng, Zhu: "A human anti-c-Met Fab fragment conjugated with doxorubicin as targeted chemotherapy for hepatocellular carcinoma." in: PLoS ONE, Vol. 8, Issue 5, pp. e63093, (2013) (PubMed).

Bin, Ma, Xu, Shi: "Embryonic hepatocyte transplantation for hepatic cirrhosis: efficacy and mechanism of action." in: World journal of gastroenterology, Vol. 18, Issue 4, pp. 309-22, (2012) (PubMed).

Chen, Wang, Guo, Yu, Burnstock, He, Xiang, Zheng: "Expression of P2Y(6) receptors in the developing mouse skeletal muscle and after injury and repair." in: Journal of anatomy, Vol. 218, Issue 6, pp. 643-51, (2011) (PubMed).

Target Details for c-MET

Target: c-MET (MET) (Met Proto-Oncogene (MET))

Alternative Name: MET

Background:

Synonyms: Hepatocyte growth factor receptor,HGF receptor,2.7.10.1,HGF/SF receptor,Proto-oncogene c-Met,Scatter factor receptor,SF receptor,Tyrosine-protein kinase Met,MET,

Tissue Specificity: Expressed in normal hepatocytes as well as in epithelial cells lining the stomach, the small and the large intestine. Found also in basal keratinocytes of esophagus and skin. High levels are found in liver, gastrointestinal tract, thyroid and kidney. Also present in the brain. .

Background: c-Met, also called MET and hepatocyte growth factor receptor (HGFR), is a protein that in humans is encoded by the MET gene. It is mapped to 7q31.2. The protein possesses tyrosine kinase activity. MET is a membrane receptor that is essential for embryonic development and wound healing. It induces several biological responses that collectively give rise to a program known as invasive growth. MET is deregulated in many types of human malignancies, including cancers of kidney, liver, stomach, breast, and brain. Normally, only stem cells and progenitor cells express MET, which allows these cells to grow invasively in order to generate new tissues in an embryo or regenerate damaged tissues in an adult. However, cancer stem cells are thought to hijack the ability of normal stem cells to express MET, and thus become the cause of cancer persistence and spread to other sites in the body.

Sequence Similarities: Belongs to the protein kinase superfamily. Tyr protein kinase family.

Molecular Weight: 154 kDa

Gene ID: 4233

UniProt: P08581

Pathways: RTK Signaling, Carbohydrate Homeostasis, Synaptic Membrane, Signaling of Hepatocyte Growth Factor Receptor