FOXA3 antibody (C-Term)

Catalog No. ABIN3042401

Product Details anti-FOXA3 Antibody

Target: FOXA3 (Forkhead Box A3 (FOXA3))

Binding Specificity: AA 291-324, C-Term

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This FOXA3 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunofluorescence (IF)

Purpose: Anti-FOXA3 Antibody Picoband®

Sequence: ELKLDAPYNF NHPFSINNLM SEQTPAPPKL DVGF

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-FOXA3 Antibody Picoband® (ABIN3042401). Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human FOXA3, different from the related mouse and rat sequences by three amino acids.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL, Human
Immunohistochemistry, 2-5 μg/mL, Human, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
Flow Cytometry(Fixed), 1-3 μg/1x10⁶ cells, Human
1. "Entrez Gene: forkhead box A3". 2. Mincheva A, Lichter P, Schütz G, Kaestner KH (February 1997). "Assignment of the human genes for hepatocyte nuclear factor 3-alpha, -beta, and -gamma (HNF3A, HNF3B, HNF3G) to 14q12-q13, 20p11, and 19q13.2-q13.4".Genomics 39 (3): 417-9. 3. Navas MA, Vaisse C, Boger S, et al. (2000). "The human HNF-3 genes: cloning, partial sequence and mutation screening in patients with impaired glucose homeostasis.". Hum. Hered. 50 (6): 370-81.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for FOXA3

Target: FOXA3 (Forkhead Box A3 (FOXA3))

Alternative Name: FOXA3 (FOXA3 Products)

Background:

Synonyms: Hepatocyte nuclear factor 3-gamma,HNF-3-gamma,HNF-3G,Fork head-related protein FKH H3,Forkhead box protein A3,Transcription factor 3G,TCF-3G,FOXA3,HNF3G, TCF3G,

Tissue Specificity: Expressed in erythroleukemia and hepatoma cell lines and in liver and pancreas. Not expressed in any other cell lines or tissues examined. .

Background: Hepatocyte nuclear factor 3-gamma (HNF-3G), also known as forkhead box protein A3 (FOXA3) or transcription factor 3G (TCF-3G), is a protein that in humans is encoded by the FOXA3 gene. This gene is mapped to 19q13.32. HNF-3G is a member of the forkhead class of DNA-binding proteins. These hepatocyte nuclear factors are transcriptional activators for liver-specific transcripts such as albumin and transthyretin, and they also interact with chromatin. Similar family members in mice have roles in the regulation of metabolism and in the differentiation of the pancreas and liver. The crystal structure of a similar protein in rat has been resolved.

Molecular Weight: 37 kDa

Gene ID: 3171

UniProt: P55318

Pathways: Carbohydrate Homeostasis