FOXO1 antibody (AA 456-655)

Catalog No. ABIN3042403

This Rabbit Polyclonal antibody specifically detects FOXO1 in WB. It exhibits reactivity toward Human and Ratand has been mentioned in 1 publication.

Quick Overview for FOXO1 antibody (AA 456-655) (ABIN3042403)

Target: FOXO1 (Forkhead Box O1 (FOXO1))

Reactivity: Human, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This FOXO1 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-FOXO1 Antibody

Binding Specificity: AA 456-655

Purpose: Anti-FOXO1A Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins

Characteristics: Anti-FOXO1A Antibody Picoband® (ABIN3042403). Tested in WB applications. This antibody reacts with Human, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human FOXO1A recombinant protein (Position: N456-G655). Human FOXO1A shares 93% amino acid (aa) sequence identity with both mouse and rat FOXO1A.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL, Rat, Human
1. Galili N, Davis RJ, Fredericks WJ, Mukhopadhyay S, Rauscher FJ, Emanuel BS, Rovera G, Barr FG (November 1993). "Fusion of a fork head domain gene to PAX3 in the solid tumour alveolar rhabdomyosarcoma". Nat. Genet. 5 (3): 230-5. doi:10.1038/ng1193-230 2. Nakae J, Kitamura T, Kitamura Y, Biggs WH, Arden KC, Accili D (January 2003). "The forkhead transcription factor Foxo1 regulates adipocyte differentiation". Dev. Cell 4 (1): 119-29. 3. Rena G, Guo S, Cichy SC, Unterman TG, Cohen P (June 1999). "Phosphorylation of the transcription factor forkhead family member FKHR by protein kinase B". J. Biol. Chem. 274 (24): 17179-83.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

References for anti-FOXO1 antibody (ABIN3042403)

Dong, Mai, Zhang, Mi, Wu, Chu, Yang, Sun: "MiR-15a/b promote adipogenesis in porcine pre-adipocyte via repressing FoxO1." in: Acta biochimica et biophysica Sinica, Vol. 46, Issue 7, pp. 565-71, (2014) (PubMed).

Target Details for FOXO1

Target: FOXO1 (Forkhead Box O1 (FOXO1))

Alternative Name: FOXO1

Background:

Synonyms: Forkhead box protein O1,Forkhead box protein O1A,Forkhead in rhabdomyosarcoma,FOXO1,FKHR, FOXO1A,

Tissue Specificity: Ubiquitous. .

Background: Forkhead box protein O1, also known as FKHR or FOXO1A, is a protein that in humans is encoded by the FOXO1 gene. It is mapped to 13q14.11. This gene belongs to the forkhead family of transcription factors which are characterized by a distinct forkhead domain. FOXO1 plays important roles in regulation of gluconeogenesis and glycogenolysis by insulin signaling, and is also central to the decision for a preadipocyte to commit to adipogenesis. This gene is primarily regulated through phosphorylation on multiple residues. Its transcriptional activity is dependent on its phosphorylation state. What's more, FOXO1 may play a role in myogenic growth and differentiation. Translocation of this gene with PAX3 has been associated with alveolar rhabdomyosarcoma.

Sequence Similarities: Contains 1 fork-head DNA-binding domain.

Molecular Weight: 70 kDa

Gene ID: 2308

UniProt: Q12778

Pathways: PI3K-Akt Signaling, Cell Division Cycle, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Carbohydrate Homeostasis, Chromatin Binding, Regulation of Carbohydrate Metabolic Process, CXCR4-mediated Signaling Events, BCR Signaling