Progesterone Receptor antibody (AA 595-933)

Catalog No. ABIN3042523

Product Details anti-Progesterone Receptor Antibody

Target: Progesterone Receptor (PGR)

Binding Specificity: AA 595-933

Reactivity: Human, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Progesterone Receptor antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Purpose: Rabbit IgG polyclonal antibody for Progesterone receptor(PGR) detection. Tested with WB, IHC-P in Human,Rat.

Brand: Picoband™

Cross-Reactivity (Details): No cross reactivity with other proteins.

Characteristics: Rabbit IgG polyclonal antibody for Progesterone receptor(PGR) detection. Tested with WB, IHC-P in Human,Rat.
Gene Name: progesterone receptor
Protein Name: Progesterone receptor

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human Progesterone Receptor recombinant protein (Position: M595-K933). Human Progesterone Receptor shares 95% and 94% amino acid (aa) sequence identity with mouse and rat Progesterone Receptor, respectively.

Isotype: IgG

Application Details

Application Notes: WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, Rat
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
Notes: Tested Species: Species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

References for anti-Progesterone Receptor antibody (ABIN3042523)

Fu, Zhao, Wu, Wu, Xu, Liu, Tong: "Therapeutic mechanisms of Tongmai Dasheng Tablet on tripterygium glycosides induced rat model for premature ovarian failure." in: Journal of ethnopharmacology, Vol. 139, Issue 1, pp. 26-33, (2011) (PubMed).

Target Details for Progesterone Receptor

Target: Progesterone Receptor (PGR)

Alternative Name: PGR (PGR Products)

Synonyms: NR3C3 antibody, PR antibody, 9930019P03Rik antibody, BB114106 antibody, ENSMUSG00000074510 antibody, PR-A antibody, PR-B antibody, XPR-1 antibody, pgr antibody, xpr-2 antibody, LOC443444 antibody, PGR antibody, pg antibody, pr antibody, gb:dq017620 antibody, progesterone receptor antibody, progesterone receptor L homeolog antibody, PGR antibody, Pgr antibody, pgr.L antibody, pgr antibody

Background: The progesterone receptor (PR) is an intracellular steroid receptor that specially binds progesterone in humans. PR has been a member of the steroid receptor superfamily. It is encoded by a single PGR gene residing on chromosome 11q22. The PGR gene uses separate promoters and translational start sites to produce 2 isoforms, PRA and PRB, which are identical except for an additional 165 amino acids present only in the N terminus of PRB. It can be observed in human breast tissues. The proteins function as dimeric molecules in nuclei to regulate the transcription of target genes in a ligand-responsive manner.

Synonyms: NR3C3 antibody|Nuclear receptor subfamily 3 group C member 3 antibody|PGR antibody|PR antibody|PRA antibody|PRB antibody|PRGR_HUMAN antibody|Progesterone receptor antibody|Progestin receptor form A antibody|Progestin receptor form B antibody

UniProt: P06401

Pathways: Nuclear Receptor Transcription Pathway, Intracellular Steroid Hormone Receptor Signaling Pathway, Steroid Hormone Mediated Signaling Pathway, Smooth Muscle Cell Migration