Caspase 8 antibody (AA 388-480)

Catalog No. ABIN3042601

This Rabbit Polyclonal antibody specifically detects Caspase 8 in WB. It exhibits reactivity toward Mouse and has been mentioned in 14+ publications.

Quick Overview for Caspase 8 antibody (AA 388-480) (ABIN3042601)

Target: Caspase 8 (CASP8)

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Caspase 8 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-Caspase 8 Antibody

Binding Specificity: AA 388-480

Purpose: Anti-Caspase-8/CASP8 Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins

Characteristics: Anti-Caspase-8/CASP8 Antibody Picoband® (ABIN3042601). Tested in WB applications. This antibody reacts with Mouse. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived mouse Caspase 8 recombinant protein (Position: S388-P480). Mouse Caspase 8 shares 83% amino acid (aa) sequence identity with human Caspase 8.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL, Mouse
1. Activated neutrophils induce epithelial cell apoptosis through oxidant-dependent tyrosine dephosphorylation of caspase-8.Jia SH, et al. Am J Pathol, 2014 Apr. 2. Intra- and interdimeric caspase-8 self-cleavage controls strength and timing of CD95-induced apoptosis.Kallenberger SM, et al. Sci Signal, 2014 Mar 11. 3. Association between CASP8 -652 6N del polymorphism (rs3834129) and colorectal cancer risk: results from a multi-centric study.Pardini B, et al. PLoS One, 2014.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

References for anti-Caspase 8 antibody (ABIN3042601)

Zhan, Hu, Yi, An, Huang: "[Corrigendum] Inhibitory activity of apogossypol in human prostate cancer in vitro and in vivo." in: Molecular medicine reports, Vol. 17, Issue 6, pp. 8010, (2018) (PubMed).

Zhou, He, Wang, Xie, Liu, Liu, Song, Ma: "Intravenous Administration Is an Effective and Safe Route for Cancer Gene Therapy Using the Bifidobacterium-Mediated Recombinant HSV-1 Thymidine Kinase and Ganciclovir." in: International journal of molecular sciences, Vol. 17, Issue 6, (2017) (PubMed).

Zhang, Huang, Wang, Luo, Wang: "2-DG-Regulated RIP and c-FLIP Effect on Liver Cancer Cell Apoptosis Induced by TRAIL." in: Medical science monitor : international medical journal of experimental and clinical research, Vol. 21, pp. 3442-8, (2016) (PubMed).

Li, Wu, Sun, Zhao, Liu, Zhang: "Croton Tiglium Extract Induces Apoptosis via Bax/Bcl-2 Pathways in Human Lung Cancer A549 Cells" in: Asian Pacific journal of cancer prevention : APJCP, Vol. 17, Issue 11, pp. 4893-4898, (2016) (PubMed).

Zhan, Hu, Yi, An, Huang: "Inhibitory activity of apogossypol in human prostate cancer in vitro and in vivo." in: Molecular medicine reports, Vol. 11, Issue 6, pp. 4142-8, (2015) (PubMed).

Liu, Chen, Wang, Yang, Xue, Zhu: "Msi1 confers resistance to TRAIL by activating ERK in liver cancer cells." in: FEBS letters, Vol. 589, Issue 8, pp. 897-903, (2015) (PubMed).

Jiang, Li, Zhou, Wang, Zhang, Wang: "Colistin-induced apoptosis in PC12 cells: involvement of the mitochondrial apoptotic and death receptor pathways." in: International journal of molecular medicine, Vol. 33, Issue 5, pp. 1298-304, (2014) (PubMed).

Yan, Liang, Zhang, Liu, Bu: "Apoptotic induction of lung adenocarcinoma A549 cells infected by recombinant RVG Newcastle disease virus (rL-RVG) in vitro." in: Molecular medicine reports, Vol. 11, Issue 1, pp. 317-26, (2014) (PubMed).

Shan, Li, Newton, Zhao, Li, Guo: "A novel protein extracted from foxtail millet bran displays anti-carcinogenic effects in human colon cancer cells." in: Toxicology letters, Vol. 227, Issue 2, pp. 129-38, (2014) (PubMed).

Xia, Luo, Lin, Zhang, Guo, He: "The effect of different treatment time of millimeter wave on chondrocyte apoptosis, caspase-3, caspase-8, and MMP-13 expression in rabbit surgically induced model of knee osteoarthritis." in: Rheumatology international, Vol. 32, Issue 9, pp. 2847-56, (2012) (PubMed).

Lin, He, Luo, Zhang, Zeng: "The effect of low-level laser to apoptosis of chondrocyte and caspases expression, including caspase-8 and caspase-3 in rabbit surgery-induced model of knee osteoarthritis." in: Rheumatology international, Vol. 32, Issue 3, pp. 759-66, (2012) (PubMed).

Zeng, Luo, Lin, Zhang, He: "The effect of therapeutic ultrasound to apoptosis of chondrocyte and caspase-3 and caspase-8 expression in rabbit surgery-induced model of knee osteoarthritis." in: Rheumatology international, Vol. 32, Issue 12, pp. 3771-7, (2012) (PubMed).

Lian, Ni, Dai, Su, Smith, Xu, He: "Sorafenib sensitizes (-)-gossypol-induced growth suppression in androgen-independent prostate cancer cells via Mcl-1 inhibition and Bak activation." in: Molecular cancer therapeutics, Vol. 11, Issue 2, pp. 416-26, (2012) (PubMed).

Guo, Luo, Zhang, Lin, Xia, He: "Comparing different physical factors on serum TNF-? levels, chondrocyte apoptosis, caspase-3 and caspase-8 expression in osteoarthritis of the knee in rabbits." in: Joint, bone, spine : revue du rhumatisme, Vol. 78, Issue 6, pp. 604-10, (2011) (PubMed).

Target Details for Caspase 8

Target: Caspase 8 (CASP8)

Alternative Name: CASP8

Background:

Synonyms: Caspase-8,CASP-8,3.4.22.61,Caspase-8 subunit p18,Caspase-8 subunit p10,Casp8,

Tissue Specificity: Expressed in a wide variety of tissues. Highest expression in spleen, thymus, lung, liver and kidney. Lower expression in heart, brain, testis and skeletal muscle.

Background: CASP8 is also known as CAP4, MACH or MCH5. This gene encodes a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes composed of a prodomain, a large protease subunit, and a small protease subunit. Activation of caspases requires proteolytic processing at conserved internal aspartic residues to generate a heterodimeric enzyme consisting of the large and small subunits. This protein is involved in the programmed cell death induced by Fas and various apoptotic stimuli. The N-terminal FADD-like death effector domain of this protein suggests that it may interact with Fas-interacting protein FADD. This protein was detected in the insoluble fraction of the affected brain region from Huntington disease patients but not in those from normal controls, which implicated the role in neurodegenerative diseases. Many alternatively spliced transcript variants encoding different isoforms have been described, although not all variants have had their full-length sequences determined.?

Sequence Similarities: Belongs to the peptidase C14A family.

Molecular Weight: 55 kDa

Gene ID: 12370

UniProt: O89110

Pathways: Apoptosis, Caspase Cascade in Apoptosis, TLR Signaling, Activation of Innate immune Response, Tube Formation, Positive Regulation of Endopeptidase Activity, Toll-Like Receptors Cascades