RUNX3 antibody (Middle Region)

Catalog No. ABIN3042652

This Rabbit Polyclonal antibody specifically detects RUNX3 in WB. It exhibits reactivity toward Mouse and Rat.

Quick Overview for RUNX3 antibody (Middle Region) (ABIN3042652)

Target: RUNX3 (Runt-Related Transcription Factor 3 (RUNX3))

Reactivity: Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RUNX3 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-RUNX3 Antibody

Binding Specificity: AA 186-205, Middle Region

Purpose: Anti-RUNX3 Antibody Picoband®

Sequence: QKIEDQTKAF PDRFGDLRMR

Cross-Reactivity (Details): No cross-reactivity with other proteins

Characteristics: Anti-RUNX3 Antibody (ABIN3042652). Tested in WB applications. This antibody reacts with Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence in the middle region of mouse RUNX3, identical to the related rat sequence.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL, Mouse, Rat
1. Brenner, O., Levanon, D., Negreanu, V., Golubkov, O., Fainaru, O., Woolf, E., Groner, Y. Loss of Runx3 function in leukocytes is associated with spontaneously developed colitis and gastric mucosal hyperplasia. Proc. Nat. Acad. Sci. 101: 16016-16021, 2004. 2. Fainaru, O., Shseyov, D., Hantisteanu, S., Groner, Y. Accelerated chemokine receptor 7-mediated dendritic cell migration in Runx3 knockout mice and the spontaneous development of asthma-like disease. Proc. Nat. Acad. Sci. 102: 10598-10603, 2005. 3. Ito, K., Liu, Q., Salto-Tellez, M., Yano, T., Tada, K., Ida, H., Huang, C., Shah, N., Inoue, M., Rajnakova, A., Hiong, K. C., Peh, B. K., Han, H. C., Ito, T., Teh, M., Yeoh, K. G., Ito, Y. RUNX3, a novel tumor suppressor, is frequently inactivated in gastric cancer by protein mislocalization. Cancer Res. 65: 7743-7750, 2005. 4. Li, Q.-L., Ito, K., Sakakura, C., Fukamachi, H., Inoue, K., Chi, X.-Z., Lee, K.-Y., Nomura, S., Lee, C.-W., Han, S.-B., Kim, H.-M., Kim, W.-J., and 15 others. Causal relationship between the loss of RUNX3 expression and gastric cancer. Cell 109: 113-124, 2002.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

Preservative: Thimerosal (Merthiolate), Sodium azide

Precaution of Use: This product contains Thimerosal (Merthiolate) and Sodium azide: POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for RUNX3

Target: RUNX3 (Runt-Related Transcription Factor 3 (RUNX3))

Alternative Name: RUNX3

Background:

Synonyms: Runt-related transcription factor 3,Acute myeloid leukemia 2 protein,Core-binding factor subunit alpha-3,CBF-alpha-3,Oncogene AML-2,Polyomavirus enhancer-binding protein 2 alpha C subunit,PEA2-alpha C,PEBP2-alpha C,SL3-3 enhancer factor 1 alpha C subunit,SL3/AKV core-binding factor alpha C subunit,Runx3,Aml2, Cbfa3, Pebp2a3,

Background: Runt-related transcription factor 3, also called AML2 is a protein that in humans is encoded by the RUNX3 gene. The RUNX3 gene encodes a Runt-related transcription factor, which is part of the RUNX gene family. By fluorescence in situ hybridization, RUNX3 was assigned to human chromosome 1p36.11. RUNX3 binds to the core site of murine Leukemia virus, the core sequences in the enhancer of the polyomavirus, and also to the enhancers of the T-cell receptor genes. It may be involved in the control of cellular proliferation and/or differentiation.

Molecular Weight: 44 kDa

UniProt: Q64131