IRAK1 antibody (N-Term)

Catalog No. ABIN3042847

Product Details anti-IRAK1 Antibody

Target: IRAK1 (Interleukin-1 Receptor-Associated Kinase 1 (IRAK1))

Binding Specificity: AA 18-37, N-Term

Reactivity: Human, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This IRAK1 antibody is un-conjugated

Application: Western Blotting (WB)

Purpose: Rabbit IgG polyclonal antibody for Interleukin-1 receptor-associated kinase 1(IRAK1) detection. Tested with WB in Human,Mouse,Rat.

Sequence: FLYEVPPWVM CRFYKVMDAL

Cross-Reactivity (Details): Predicted Cross Reactivity: mouse
No cross reactivity with other proteins.
Predicted Cross Reactivity: Species predicted to be fit for the product based on sequence similarities.

Characteristics: Rabbit IgG polyclonal antibody for Interleukin-1 receptor-associated kinase 1(IRAK1) detection. Tested with WB in Human,Mouse,Rat.
Gene Name: interleukin-1 receptor-associated kinase 1
Protein Name: Interleukin-1 receptor-associated kinase 1

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the N-terminus of human IRAK(18-37aa FLYEVPPWVMCRFYKVMDAL), identical to the related rat and mouse sequences.

Isotype: IgG

Application Details

Application Notes: WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, Rat, Predicted Species: Mouse
Notes: Tested Species: Species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities.
Other applications have not been tested. Optimal dilutions should be determined by end users.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

Preservative: Thimerosal (Merthiolate), Sodium azide

Precaution of Use: This product contains Sodium azide and Thimerosal (Merthiolate): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

Expiry Date: 12 months

References for anti-IRAK1 antibody (ABIN3042847)

Liu, Wang, Qian, Li: "Xuebijing exerts protective effects on lung permeability leakage and lung injury by upregulating Toll-interacting protein expression in rats with sepsis." in: International journal of molecular medicine, Vol. 34, Issue 6, pp. 1492-504, (2014) (PubMed).

Target Details for IRAK1

Target: IRAK1 (Interleukin-1 Receptor-Associated Kinase 1 (IRAK1))

Alternative Name: IRAK1 (IRAK1 Products)

Synonyms: IRAK1 antibody, IRAK antibody, pelle antibody, AA408924 antibody, IRAK-1 antibody, IRAK1-S antibody, Il1rak antibody, Plpk antibody, mPLK antibody, RGD1563841 antibody, interleukin 1 receptor associated kinase 1 antibody, interleukin-1 receptor-associated kinase 1 antibody, IRAK1 antibody, irak1 antibody, Irak1 antibody

Background: Interleukin-1 receptor-associated kinase 1, also called IRAK1, is an enzyme that in humans is encoded by the IRAK1 gene. By radiation hybrid analysis, this gene is mapped to chromosome Xq28. Serine/threonine-protein kinase plays a critical role in initiating innate immune response against foreign pathogens. This gene involved in Toll-like receptor(TLR) and IL-1R signaling pathways. This gene encodes the interleukin-1 receptor-associated kinase 1, one of two putative serine/threonine kinases that become associated with the interleukin-1 receptor(IL1R) upon stimulation. This gene is partially responsible for IL1-induced upregulation of the transcription factor NF-kappa B.

Synonyms: Il1rak antibody|Interleukin 1 receptor associated kinase 1 antibody|Interleukin-1 receptor-associated kinase 1 antibody|IRAK antibody|IRAK-1 antibody|Irak1 antibody|IRAK1_HUMAN antibody|mPLK antibody|OTTHUMP00000026014 antibody|OTTHUMP00000026015 antibody|OTTHUMP00000026020 antibody|OTTHUMP00000180621 antibody|Pelle antibody|Pelle homolog antibody|Pelle-like protein kinase antibody|Plpk antibody

UniProt: P51617

Pathways: NF-kappaB Signaling, TLR Signaling, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Toll-Like Receptors Cascades