CRTC1 antibody (C-Term)

Catalog No. ABIN3042965

Product Details anti-CRTC1 Antibody

Target: CRTC1 (CREB Regulated Transcription Coactivator 1 (CRTC1))

Binding Specificity: AA 506-521, C-Term

Reactivity: Human, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CRTC1 antibody is un-conjugated

Application: Western Blotting (WB)

Purpose: Rabbit IgG polyclonal antibody for CREB-regulated transcription coactivator 1(CRTC1) detection. Tested with WB in Human,Rat.

Sequence: EQQMAARQAN ALSHQL

Cross-Reactivity (Details): No cross reactivity with other proteins.

Characteristics: Rabbit IgG polyclonal antibody for CREB-regulated transcription coactivator 1(CRTC1) detection. Tested with WB in Human,Rat.
Gene Name: CREB regulated transcription coactivator 1
Protein Name: CREB-regulated transcription coactivator 1

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human TORC1(506-521aa EQQMAARQANALSHQL), different from the related rat and mouse sequences by two amino acids.

Isotype: IgG

Application Details

Application Notes: WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, Rat, The detection limit for CRTC1 is approximately 1 ng/lane under reducing conditions.
Notes: Tested Species: Species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities.
Other applications have not been tested. Optimal dilutions should be determined by end users.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

Preservative: Thimerosal (Merthiolate), Sodium azide

Precaution of Use: This product contains Sodium azide and Thimerosal (Merthiolate): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for CRTC1

Target: CRTC1 (CREB Regulated Transcription Coactivator 1 (CRTC1))

Alternative Name: CRTC1 (CRTC1 Products)

Synonyms: MECT1 antibody, TORC-1 antibody, TORC1 antibody, WAMTP1 antibody, AI413414 antibody, Mect1 antibody, R74955 antibody, mKIAA0616 antibody, CRTC1 antibody, crtc1 antibody, fc68c05 antibody, pde4cb antibody, si:ch211-255d18.1 antibody, si:ch211-255d18.8 antibody, wu:fc68c05 antibody, CREB regulated transcription coactivator 1 antibody, CREB regulated transcription coactivator 1b antibody, CRTC1 antibody, Crtc1 antibody, crtc1 antibody, crtc1b antibody

Background: CRTC1(CREB-Regulated Transcription Coactivator 1), also known as MECT1, TORC1 or KIAA0616, is a protein that in humans is encoded by the CRTC1 gene. By sequence analysis, Tonon et al.(2003) mapped the CRTC1 gene to chromosome 19p13. Kovacs et al.(2007) found that Torc1 was expressed in adult mouse brain and cultured neurons, and that it translocated to the nucleus upon concomitant activation of calcium and cAMP signaling pathways. Mair et al.(2011) demonstrated that CRTC1 is a direct AMPK target, and interacts with the CREB homolog-1(CRH1) transcription factor in vivo. The prolongevity effects of activating AMPK or deactivating calcineurin decrease CRTC1 and CRH1 activity and induce transcriptional responses similar to those of CRH1-null worms.

Synonyms: KIAA0616 antibody|CREB regulated transcription coactivator 1 antibody|CREB-regulated transcription coactivator 1 antibody|CRTC1 antibody|CRTC1_HUMAN antibody|FLJ14027 antibody|MECT 1 antibody|Mucoepidermoid carcinoma translocated 1 antibody|Mucoepidermoid carcinoma translocated protein 1 antibody|TORC-1 antibody|TORC1 antibody|Transducer of CREB protein 1 antibody|Transducer of regulated cAMP response element binding protein 1 antibody|Transducer of regulated cAMP response element-binding protein(CREB) 1 antibody|Transducer of regulated cAMP response element-binding protein 1 antibody|WAMTP1 antibody