CRTC1 antibody (C-Term)

Catalog No. ABIN3042965

The Rabbit Polyclonal anti-CRTC1 antibody is suitable to detect CRTC1 in samples from Human and Rat. It has been validated for WB.

Quick Overview for CRTC1 antibody (C-Term) (ABIN3042965)

Target: CRTC1 (CREB Regulated Transcription Coactivator 1 (CRTC1))

Reactivity: Human, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CRTC1 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-CRTC1 Antibody

Binding Specificity: C-Term

Purpose: Anti-TORC1/CRTC1 Antibody

Sequence: EQQMAARQAN ALSHQL

Specificity: No cross reactivity with other proteins.

Cross-Reactivity (Details): No cross-reactivity with other proteins

Predicted Reactivity: different from the related rat,mouse sequences by two amino acids.

Characteristics: Anti-TORC1/CRTC1 Antibody. Tested in WB applications. This antibody reacts with Human, Rat.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human TORC1, different from the related rat and mouse sequences by two amino acids.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL, Human, Rat

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains antibody formulated with stabilizing components, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

Preservative: Thimerosal (Merthiolate), Sodium azide

Precaution of Use: This product contains Thimerosal (Merthiolate) and Sodium azide: POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for CRTC1

Target: CRTC1 (CREB Regulated Transcription Coactivator 1 (CRTC1))

Alternative Name: CRTC1

Background:

Background: CRTC1 (CREB-Regulated Transcription Coactivator 1), also known as MECT1, TORC1 or KIAA0616, is a protein that in humans is encoded by the CRTC1 gene. By sequence analysis, Tonon et al. (2003) mapped the CRTC1 gene to chromosome 19p13. Kovacs et al. (2007) found that Torc1 was expressed in adult mouse brain and cultured neurons, and that it translocated to the nucleus upon concomitant activation of calcium and cAMP signaling pathways. Mair et al. (2011) demonstrated that CRTC1 is a AMPK target, and interacts with the CREB homolog-1 (CRH1) transcription factor in vivo. The prolongevity effects of activating AMPK or deactivating calcineurin decrease CRTC1 and CRH1 activity and induce transcriptional responses similar to those of CRH1-null worms.

Gene Full Name: CREB regulated transcription coactivator 1

Sequence Similarities: Belongs to the TORC family.

Molecular Weight: 78 kDa

Gene ID: 23373

UniProt: Q6UUV9