ASIC3 antibody (N-Term)

Catalog No. ABIN3042990

This anti-ASIC3 antibody is a Rabbit Polyclonal antibody detecting ASIC3 in WB and FACS. Suitable for Human, Rat and Mouse. This Primary Antibody has been cited in 1 publication.

Quick Overview for ASIC3 antibody (N-Term) (ABIN3042990)

Target: ASIC3 (ACCN3) (Amiloride-Sensitive Cation Channel 3 (ACCN3))

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ASIC3 antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS)

Product Details anti-ASIC3 Antibody

Binding Specificity: AA 56-73, N-Term

Purpose: Anti-Acid-sensing ion channel 3 ASIC3 Antibody Picoband®

Sequence: FLYQVAERVR YYREFHHQ

Cross-Reactivity (Details): No cross-reactivity with other proteins

Characteristics: Anti-Acid-sensing ion channel 3 ASIC3 Antibody (ABIN3042990). Tested in Flow Cytometry, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the N-terminus of human ASIC3, different from the related rat and mouse sequences by two amino acids.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL, Human, Mouse, Rat
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human1. Babinski, K., Le, K.-T., Seguela, P. Molecular cloning and regional distribution of a human proton receptor subunit with biphasic functional properties. J. Neurochem. 72: 51-57, 1999. 2. Birdsong, W. T., Fierro, L., Williams, F. G., Spelta, V., Naves, L. A., Knowles, M., Marsh-Haffner, J., Adelman, J. P., Almers, W., Elde, R. P., McCleskey, E. W. Sensing muscle ischemia: coincident detection of acid and ATP via interplay of two ion channels. Neuron 68: 739-749, 2010. 3. de Weille, J. R., Bassilana, F., Lazdunski, M., Waldmann, R. Identification, functional expression and chromosomal localisation of a sustained human proton-gated cation channel. FEBS Lett. 433: 257-260, 1998.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

Preservative: Thimerosal (Merthiolate), Sodium azide

Precaution of Use: This product contains Thimerosal (Merthiolate) and Sodium azide: POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

References for anti-ASIC3 antibody (ABIN3042990)

Schuhmacher, Callejo, Srivats, Smith: "Naked mole-rat acid-sensing ion channel 3 forms nonfunctional homomers, but functional heteromers." in: The Journal of biological chemistry, Vol. 293, Issue 5, pp. 1756-1766, (2018) (PubMed).

Target Details for ASIC3

Target: ASIC3 (ACCN3) (Amiloride-Sensitive Cation Channel 3 (ACCN3))

Alternative Name: ASIC3

Background:

Synonyms: Acid-sensing ion channel 3,ASIC3,hASIC3,Amiloride-sensitive cation channel 3,Neuronal amiloride-sensitive cation channel 3,Testis sodium channel 1,hTNaC1,ASIC3,ACCN3, SLNAC1, TNAC1,

Tissue Specificity: Expressed by sensory neurons. Strongly expressed in brain, spinal chord, lung, lymph nodes, kidney, pituitary, heart and testis. .

Background: ASIC3 (Acid-Sensing Ion Channel3), also known as TESTIS SODIUM CHANNEL 1 (TNAC1) or DORSAL ROOT ACID-SENSING ION CHANNEL (DRASIC), is a protein that in humans is encoded by the ASIC3 gene. ASIC3 belongs to a family of acid-sensing channel proteins that are structurally related to epithelial sodium channel proteins and support acid-activated membrane currents. By radiation hybrid analysis, de Weille et al. (1998) mapped the ACCN3 gene to chromosome 7q35. De Weille et al. (1998) found that human ASIC3 supported an H (+)-gated cation current in COS cells with kinetics similar to those of rat Asic3. Babinski et al. (1999) expressed homomeric human ASIC3 channels in Xenopus oocytes and found that rapid reduction in extracellular pH resulted in a biphasic response characterized by a fast and rapidly desensitizing current followed by a slow and sustained current that returned to baseline only on return to physiologic pH .

Sequence Similarities: Belongs to the amiloride-sensitive sodium channel (TC 1.A.6) family. ASIC3 subfamily.

Molecular Weight: 69 kDa