E2F2 antibody (C-Term)

Catalog No. ABIN3043044

Product Details anti-E2F2 Antibody

Target: E2F2 (E2F Transcription Factor 2 (E2F2))

Binding Specificity: AA 422-427, C-Term

Reactivity: Human, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This E2F2 antibody is un-conjugated

Application: Western Blotting (WB)

Purpose: Rabbit IgG polyclonal antibody for Transcription factor E2F2(E2F2) detection. Tested with WB in Human,Mouse,Rat.

Sequence: ISDLFDSYDL GDLLIN

Cross-Reactivity (Details): Predicted Cross Reactivity: mouse
No cross reactivity with other proteins.
Predicted Cross Reactivity: Species predicted to be fit for the product based on sequence similarities.

Characteristics: Rabbit IgG polyclonal antibody for Transcription factor E2F2(E2F2) detection. Tested with WB in Human,Mouse,Rat.
Gene Name: E2F transcription factor 2
Protein Name: Transcription factor E2F2(E2F-2)

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human E2F2(422-427aa ISDLFDSYDLGDLLIN), identical to the related mouse sequence.

Isotype: IgG

Application Details

Application Notes: WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, Rat, Predicted Species: Mouse
Notes: Tested Species: Species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities.
Other applications have not been tested. Optimal dilutions should be determined by end users.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

Preservative: Thimerosal (Merthiolate), Sodium azide

Precaution of Use: This product contains Sodium azide and Thimerosal (Merthiolate): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

Expiry Date: 12 months

References for anti-E2F2 antibody (ABIN3043044)

Liu, Zhang, Lan, Wang, Mou, Shao, Liu, Yang, Lin, Lin, Ji: "GCN5 Potentiates Glioma Proliferation and Invasion via STAT3 and AKT Signaling Pathways." in: International journal of molecular sciences, Vol. 16, Issue 9, pp. 21897-910, (2016) (PubMed).

Target Details for E2F2

Target: E2F2 (E2F Transcription Factor 2 (E2F2))

Alternative Name: E2F2 (E2F2 Products)

Synonyms: E2F-2 antibody, CG1071 antibody, Dmel\\CG1071 antibody, E2F antibody, E2F2 antibody, dE2F-2 antibody, dE2F2 antibody, dE2f2 antibody, de2f2 antibody, e2f2 antibody, 9230110J10 antibody, E130207A07 antibody, fb40b03 antibody, wu:fb40b03 antibody, E2F transcription factor 2 antibody, si:ch211-160f23.5 antibody, E2F2 antibody, E2f2 antibody, si:ch211-160f23.5 antibody

Background: E2F2(E2F transcription factor 2) also called E2F-2, is a member of the E2F family of transcription factors. The E2F family plays a crucial role in the control of cell cycle and action of tumor suppressor proteins and is also a target of the transforming proteins of small DNA tumor viruses. The E2F2 gene is mapped to 1p36 by fluorescence in situ hybridization. Electrophoretic mobility shift assays revealed the specific binding of E2F2 to ECE1b promoter sequences containing either allele of the C-338A polymorphism, with the -338A allele being associated with an increased affinity to E2F2 compared with -338C. The ability of Myc to induce S phase was impaired in the absence of either E2f2 or E2f3 but not E2f1 or E2f4. In contrast, the ability of Myc to induce apoptosis was markedly reduced in cells deleted for E2f1 but not E2f2 or E2f3.

Synonyms: dE2F2 antibody|E2F transcription factor 2 antibody|E2F-2 antibody|E2F2 antibody|E2F2_HUMAN antibody|Transcription factor E2F2 antibody

UniProt: Q14209

Pathways: Cell Division Cycle, Mitotic G1-G1/S Phases, DNA Replication