CYGB antibody (N-Term)

Catalog No. ABIN3043127

Product Details anti-CYGB Antibody

Target: CYGB (Cytoglobin (CYGB))

Binding Specificity: AA 50-68, N-Term

Reactivity: Human, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CYGB antibody is un-conjugated

Application: Western Blotting (WB)

Purpose: Rabbit IgG polyclonal antibody for Cytoglobin(CYGB) detection. Tested with WB in Human,Mouse,Rat.

Sequence: FVNFPSAKQY FSQFKHMED

Cross-Reactivity (Details): Predicted Cross Reactivity: mouse
No cross reactivity with other proteins.
Predicted Cross Reactivity: Species predicted to be fit for the product based on sequence similarities.

Characteristics: Rabbit IgG polyclonal antibody for Cytoglobin(CYGB) detection. Tested with WB in Human,Mouse,Rat.
Gene Name: cytoglobin
Protein Name: Cytoglobin

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the N-terminus of human Cytoglobin(50-68aa FVNFPSAKQYFSQFKHMED), identical to the related rat sequence, different from the related mouse sequence by one amino acid.

Isotype: IgG

Application Details

Application Notes: WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, Rat, Predicted Species: Mouse
Notes: Tested Species: Species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities.
Other applications have not been tested. Optimal dilutions should be determined by end users.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

Preservative: Thimerosal (Merthiolate), Sodium azide

Precaution of Use: This product contains Sodium azide and Thimerosal (Merthiolate): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for CYGB

Target: CYGB (Cytoglobin (CYGB))

Alternative Name: CYGB (CYGB Products)

Synonyms: cygb antibody, wu:fb77d07 antibody, wu:fb93a03 antibody, zgc:109806 antibody, MGC89562 antibody, CYGB antibody, cygb-1 antibody, MGC84306 antibody, 3110001K20Rik antibody, HGb antibody, Staap antibody, HGB antibody, STAP antibody, Stap antibody, cytoglobin 1 antibody, cytoglobin antibody, cytoglobin-1 antibody, cytoglobin L homeolog antibody, cygb1 antibody, cygb antibody, CYGB antibody, cygb.L antibody, Cygb antibody

Background: Cytoglobin(CYGB), also called HGB or STAP, is a ubiquitously expressed hexacoordinate hemoglobin that may facilitate diffusion of oxygen through tissues, scavenge nitric oxide or other reactive oxygen species, or serve a protective function during oxidative stress. The cytoglobin gene is mapped on 17q25.1. The CYGB gene contains 4 exons and spans about 9 kb. Cytoglobin has many elements common to vertebrate globins, including invariant histidine residues, and the amino acids that form the heme pocket share similarity with pentacoordinate myoglobin. In contrast to the high oxygen affinities displayed by most hexacoordinate hemoglobins, the characteristics of CYGB indicate that it can facilitate oxygen transport. Because the oxygen affinity of CYGB is more similar to myoglobin than to neuroglobin, and the oxy form of CYGB resists autooxidation, CYGB is proposed to represent a tissue oxygen reservoir by Sawai et al.

Synonyms: Cygb antibody|CYGB_HUMAN antibody|Cytoglobin antibody|Hgb antibody|Histoglobin antibody|STAP antibody|Stellate cell activation associated protein antibody|Stellate cell activation-associated protein antibody