MAPT antibody (N-Term)

Catalog No. ABIN3043190

The Rabbit Polyclonal anti-MAPT antibody has been validated for WB. It is suitable to detect MAPT in samples from Human, Rat and Mouse. There are 7+ publications available.

Quick Overview for MAPT antibody (N-Term) (ABIN3043190)

Target: MAPT (Microtubule-Associated Protein tau (MAPT))

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MAPT antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-MAPT Antibody

Binding Specificity: AA 29-44, N-Term

Purpose: Anti-Tau/MAPT Antibody Picoband®

Sequence: YTMHQDQEGD TDAGLK

Cross-Reactivity (Details): No cross-reactivity with other proteins

Characteristics: Anti-Tau/MAPT Antibody (ABIN3043190). Tested in WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the N-terminus of human Tau, different from the related mouse and rat sequences by three amino acids.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL, Human, Mouse, Rat
1. Andreadis, A., Brown, W. M., Kosik, K. S.Structure and novel exons of the human tau gene.Biochemistry 31: 10626-10633, 1992. 2. Donlon, T. A., Harris, P., Neve, R. L.Localization of microtubule-associated protein tau (MTBT1) to chromosome 17q21. (Abstract)Cytogenet. Cell Genet. 46: 607, 1987. 3. Rademakers, R., Cruts, M., van Broeckhoven, C.The role of tau (MAPT) in frontotemporal dementia and related tauopathies.Hum. Mutat. 24: 277-295, 2004.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

Preservative: Thimerosal (Merthiolate), Sodium azide

Precaution of Use: This product contains Thimerosal (Merthiolate) and Sodium azide: POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

References for anti-MAPT antibody (ABIN3043190)

Tang, Yu, Wu, Chen, Zeng, Wang, Yang, Mei, Cao, Qin: "Lychee seed extract protects against neuronal injury and improves cognitive function in rats with type II diabetes mellitus with cognitive impairment." in: International journal of molecular medicine, Vol. 41, Issue 1, pp. 251-263, (2018) (PubMed).

Zhou, Liu, Yuan, Lu: "The expression of insulin-like growth factor-1 in senior patients with diabetes and dementia." in: Experimental and therapeutic medicine, Vol. 13, Issue 1, pp. 103-106, (2017) (PubMed).

Zhu, Li, Yuan, Li, Tian, Jia, Xiao: "Allicin improves endoplasmic reticulum stress-related cognitive deficits via PERK/Nrf2 antioxidative signaling pathway." in: European journal of pharmacology, Vol. 762, pp. 239-46, (2015) (PubMed).

Li, Li, Li, Quan, Wang: "Cellular and molecular mechanisms underlying the action of ginsenoside Rg1 against Alzheimer's disease." in: Neural regeneration research, Vol. 7, Issue 36, pp. 2860-6, (2014) (PubMed).

Tu, Pang, Chen, Zhang, Zhang, Lu, Wang: "Effects of surface charges of graphene oxide on neuronal outgrowth and branching." in: The Analyst, Vol. 139, Issue 1, pp. 105-15, (2013) (PubMed).

Wang, Feng, Fu, Li: "Panax notoginsenoside Rb1 ameliorates Alzheimer's disease by upregulating brain-derived neurotrophic factor and downregulating Tau protein expression." in: Experimental and therapeutic medicine, Vol. 6, Issue 3, pp. 826-830, (2013) (PubMed).

Tan, Cao, Wang, Lv, Wu, Ma: "Protective effects of lithium treatment for spatial memory deficits induced by tau hyperphosphorylation in splenectomized rats." in: Clinical and experimental pharmacology & physiology, Vol. 37, Issue 10, pp. 1010-5, (2010) (PubMed).

Target Details for MAPT

Target: MAPT (Microtubule-Associated Protein tau (MAPT))

Alternative Name: MAPT

Background:

Synonyms: Microtubule-associated protein tau,Neurofibrillary tangle protein,Paired helical filament-tau,PHF-tau,MAPT,MAPTL, MTBT1, TAU,

Tissue Specificity: Expressed in neurons. Isoform PNS-tau is expressed in the peripheral nervous system while the others are expressed in the central nervous system.

Background: MAPT, Microtubule-associated protein tau, appears to be enriched in axons. The MAPT gene is assigned to chromosome 17 by hybridization of a cDNA clone to flow-sorted and spot-blotted chromosomes and to 17q21 by in situ hybridization, containing 16 exons. The tau proteins are the product of alternative splicingfrom a single gene that in humans is designated MAPT. Tau proteins are proteins that stabilize microtubules. They are abundant in neurons in the central nervous system and are less common elsewhere. When tau proteins are defective, and no longer stabilize microtubules properly, they can result in dementias such as Alzheimer's disease.

Sequence Similarities: Contains 4 Tau/MAP repeats.

Molecular Weight: 79 kDa

UniProt: P10636

Pathways: MAPK Signaling, Microtubule Dynamics, M Phase, Regulation of Cell Size