CD11b antibody (AA 17-382)

Catalog No. ABIN3043258

Product Details anti-CD11b Antibody

Target: CD11b (ITGAM) (Integrin alpha M (ITGAM))

Binding Specificity: AA 17-382

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CD11b antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunohistochemistry (IHC), Western Blotting (WB)

Purpose: Anti-CD11b/ITGAM Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-CD11b/ITGAM Antibody Picoband® (ABIN3043258). Tested in Flow Cytometry, IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human CD11b recombinant protein (Position: F17-T382). Human CD11b shares 79% and 61% amino acid (aa) sequences identity with mouse and rat CD11b, respectively.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL, Human
Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human, Mouse, Rat
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
1. Arnaout, M. A., Remold-O'Donnell, E., Pierce, M. W., Harris, P., Tenen, D. G. Molecular cloning of the alpha-subunit of human and guinea pig leukocyte adhesion glycoprotein Mo1: chromosomal localization and homology to the alpha-subunits of integrins. Proc. Nat. Acad. Sci. 85: 2776-2780, 1988. 2. Solovjov D, Pluskota E, Plow E (2005). "Distinct roles for the alpha and beta subunits in the functions of integrin alphaMbeta2". J Biol Chem 280 (2): 1336-45. 3. Simon, D. I., Chen, Z., Seifert, P., Edelman, E. R., Ballantyne, C. M., Rogers, C. Decreased neointimal formation in Mac-1 -/- mice reveals a role for inflammation in vascular repair after angioplasty. J. Clin. Invest. 105: 293-300, 2000.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

References for anti-CD11b antibody (ABIN3043258)

Mu, Jing, Guo: "Expressions of CD11a, CD11b, and CD11c integrin proteins in rats with myocardial hypertrophy." in: Iranian journal of basic medical sciences, Vol. 17, Issue 11, pp. 874-8, (2015) (PubMed).

Ren, Zhang, Zhou, Sun, Zhang, Wei, Zhang, Si: "Tanshinone IIA prevents the loss of nigrostriatal dopaminergic neurons by inhibiting NADPH oxidase and iNOS in the MPTP model of Parkinson's disease." in: Journal of the neurological sciences, Vol. 348, Issue 1-2, pp. 142-52, (2015) (PubMed).

Zhao, Wang, Wang, Sun, Yang, Qiu, Wu, Zhang, Li, Zhang, Zhao, Zou, Ge: "CXCR6 deficiency ameliorated myocardial ischemia/reperfusion injury by inhibiting infiltration of monocytes and IFN-?-dependent autophagy." in: International journal of cardiology, Vol. 168, Issue 2, pp. 853-62, (2013) (PubMed).

Yuan, Su, Pu, Liu, Chen, Zhu, Zhu, Zhang, He: "Ethyl pyruvate promotes spinal cord repair by ameliorating the glial microenvironment." in: British journal of pharmacology, Vol. 166, Issue 2, pp. 749-63, (2012) (PubMed).

Leng, Zhan, Wang, Liu, Gong, Gao, Wu, Wang, Zhao, Wang, Zhang, Pang, Qian: "Anti-heat shock protein 70 autoantibody epitope changes and BD091 promotes atherosclerosis in rats." in: Cell stress & chaperones, Vol. 15, Issue 6, pp. 947-58, (2011) (PubMed).

Shi, Xun, Yue, Zhang, Ren, Wang, Wu, Shi, Yang, Lei: "Cloning, characterization, and expression analysis of goat (Capra hircus) phospholipid hydroperoxide glutathione peroxidase (PHGPx)." in: International journal of biological sciences, Vol. 6, Issue 4, pp. 316-26, (2010) (PubMed).

Target Details for CD11b

Target: CD11b (ITGAM) (Integrin alpha M (ITGAM))

Alternative Name: ITGAM (ITGAM Products)

Background:

Synonyms: Integrin alpha-M,CD11 antigen-like family member B,CR-3 alpha chain,Cell surface glycoprotein MAC-1 subunit alpha,Leukocyte adhesion receptor MO1,Neutrophil adherence receptor,CD11b,ITGAM,CD11B, CR3A,

Tissue Specificity: Predominantly expressed in monocytes and granulocytes.

Background: Integrin alpha M (ITGAM) is one protein subunit that forms the heterodimeric integrin alpha-M beta-2 (αMβ2) molecule, also known as macrophage-1 antigen (Mac-1) or complement receptor 3 (CR3). It is mapped to 16p11.2. ITGAM has a role in vascular repair after mechanical arterial injury. It is implicated in various adhesive interactions of monocytes, macrophages and granulocytes as well as in mediating the uptake of complement-coated particles. What's more, ITGAM probably recognizes the R-G-D peptide in C3b, and it is also a receptor for fibrinogen, factor X and ICAM1.

Sequence Similarities: Belongs to the integrin alpha chain family.

Molecular Weight: 45 kDa

Gene ID: 3684

UniProt: P11215

Pathways: Apoptosis, Activation of Innate immune Response, Toll-Like Receptors Cascades, Activated T Cell Proliferation