KEAP1 antibody (AA 25-205)
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- Target See all KEAP1 Antibodies
- KEAP1 (Kelch-Like ECH-Associated Protein 1 (KEAP1))
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Binding Specificity
- AA 25-205
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Reactivity
- Human
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- This KEAP1 antibody is un-conjugated
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Application
- Western Blotting (WB)
- Purpose
- Rabbit IgG polyclonal antibody for Kelch-like ECH-associated protein 1(KEAP1) detection. Tested with WB in Human.
- Cross-Reactivity (Details)
- No cross reactivity with other proteins.
- Characteristics
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Rabbit IgG polyclonal antibody for Kelch-like ECH-associated protein 1(KEAP1) detection. Tested with WB in Human.
Gene Name: kelch-like ECH-associated protein 1
Protein Name: Kelch-like ECH-associated protein 1 - Purification
- Immunogen affinity purified.
- Immunogen
- E.coli-derived human Keap1 recombinant protein (Position: E25-E205). Human Keap1 shares 96.7% and 97.2% amino acid (aa) sequence identity with mouse and rat Keap1, respectively.
- Isotype
- IgG
- Top Product
- Discover our top product KEAP1 Primary Antibody
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- Application Notes
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WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human
Notes: Tested Species: Species with positive results.
Other applications have not been tested. Optimal dilutions should be determined by end users. - Comment
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Antibody can be supported by chemiluminescence kit ABIN921124 in WB.
- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
- Concentration
- 500 μg/mL
- Buffer
- Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
- Avoid repeated freezing and thawing.
- Storage
- 4 °C/-20 °C
- Storage Comment
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At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.
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Bone marrow mesenchymal stem cells protect against bleomycin-induced pulmonary fibrosis in rat by activating Nrf2 signaling." in: International journal of clinical and experimental pathology, Vol. 8, Issue 7, pp. 7752-61, (2016) (PubMed).
: "Melatonin antagonizes Mn-induced oxidative injury through the activation of keap1-Nrf2-ARE signaling pathway in the striatum of mice." in: Neurotoxicity research, Vol. 27, Issue 2, pp. 156-71, (2015) (PubMed).
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Bone marrow mesenchymal stem cells protect against bleomycin-induced pulmonary fibrosis in rat by activating Nrf2 signaling." in: International journal of clinical and experimental pathology, Vol. 8, Issue 7, pp. 7752-61, (2016) (PubMed).
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- Target
- KEAP1 (Kelch-Like ECH-Associated Protein 1 (KEAP1))
- Alternative Name
- KEAP1 (KEAP1 Products)
- Background
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KEAP1 (KELCH-LIKE ECH-ASSOCIATED PROTEIN 1), is a protein that in humans is encoded by the Keap1 gene. The KIAA0132 gene is mapped on 19p13.2. Keap1 contains a central BTB/POZ domain and a C-terminal double glycine repeat (DGR), or Kelch, module. Keap1 has been shown to interact with Nrf2, a master regulator of the antioxidant response, which is important for the amelioration of oxidative stress. In the presence of the electrophilic agent diethylmalate, Nrf2 activity is released from Keap1 and Nrf2 translocate to the nucleus. Under quiescent conditions, Nrf2 is anchored in the cytoplasm through binding to Keap1, which, in turn, facilitates the ubiquitination and subsequent proteolysis of Nrf2. Because Nrf2 activation leads to a coordinated antioxidant and anti-inflammatory response, and Keap1 represses Nrf2 activation, Keap1 has become a very attractive drug target.
Synonyms: Cytosolic inhibitor of Nrf2 antibody|INrf 2 antibody|INrf2 antibody|Keap 1 antibody|KEAP1 antibody|KEAP1_HUMAN antibody|Kelch like ECH associated protein 1 antibody|Kelch like family member 19 antibody|Kelch like protein 19 antibody|Kelch-like ECH-associated protein 1 antibody|Kelch-like protein 19 antibody|KIAA0132 antibody|KLHL 19 antibody|KLHL19 antibody|MGC10630 antibody|MGC1114 antibody|MGC20887 antibody|MGC4407 antibody|MGC9454 antibody - Gene ID
- 9817
- UniProt
- Q14145
- Pathways
- Maintenance of Protein Location
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