KEAP1 antibody (AA 25-205)

Catalog No. ABIN3043275

Cited in 2+ publications. The Rabbit Polyclonal anti-KEAP1 antibody (ABIN3043275) specifically detects KEAP1 in WB. The antibody is reactive with Human samples.

Quick Overview for KEAP1 antibody (AA 25-205) (ABIN3043275)

Target: KEAP1 (Kelch-Like ECH-Associated Protein 1 (KEAP1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This KEAP1 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-KEAP1 Antibody

Binding Specificity: AA 25-205

Purpose: Anti-Keap1 Antibody

Specificity: No cross reactivity with other proteins.

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Predicted Reactivity: Human Keap1 shares 96.7%,97.2% amino acid (aa) sequence identity with mouse,rat Keap1,respectively.

Characteristics: Anti-Keap1 Antibody. Tested in WB applications. This antibody reacts with Human.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human Keap1 recombinant protein (Position: E25-E205). Human Keap1 shares 96.7% and 97.2% amino acid (aa) sequence identity with mouse and rat Keap1, respectively.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL, Human

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains antibody formulated with stabilizing components, 0.9 mg NaCl, 0.2 mg Na2HPO4, and 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

References for anti-KEAP1 antibody (ABIN3043275)

Ni, Wang, Qiu, Pang, Song, Guo: "Bone marrow mesenchymal stem cells protect against bleomycin-induced pulmonary fibrosis in rat by activating Nrf2 signaling." in: International journal of clinical and experimental pathology, Vol. 8, Issue 7, pp. 7752-61, (2016) (PubMed).

Deng, Zhu, Mi, Xu, Jiao, Li, Xu, Liu, Xu: "Melatonin antagonizes Mn-induced oxidative injury through the activation of keap1-Nrf2-ARE signaling pathway in the striatum of mice." in: Neurotoxicity research, Vol. 27, Issue 2, pp. 156-71, (2015) (PubMed).

Target Details for KEAP1

Target: KEAP1 (Kelch-Like ECH-Associated Protein 1 (KEAP1))

Alternative Name: KEAP1

Background:

Background: KEAP1 (KELCH-LIKE ECH-ASSOCIATED PROTEIN 1), is a protein that in humans is encoded by the Keap1 gene. The KIAA0132 gene is mapped on 19p13.2. Keap1 contains a central BTB/POZ domain and a C-terminal double glycine repeat (DGR), or Kelch, module. Keap1 has been shown to interact with Nrf2, a master regulator of the antioxidant response, which is important for the amelioration of oxidative stress. In the presence of the electrophilic agent diethylmalate, Nrf2 activity is released from Keap1 and Nrf2 translocate to the nucleus. Under quiescent conditions, Nrf2 is anchored in the cytoplasm through binding to Keap1, which, in turn, facilitates the ubiquitination and subsequent proteolysis of Nrf2. Because Nrf2 activation leads to a coordinated antioxidant and anti-inflammatory response, and Keap1 represses Nrf2 activation, Keap1 has become a very attractive drug target.

Gene Full Name: kelch like ECH associated protein 1

Molecular Weight: 66 kDa

Gene ID: 9817

UniProt: Q14145

Pathways: Maintenance of Protein Location